Core--Cellular and Molecular Biology

核心--细胞与分子生物学

• 批准号: 7063101
• 负责人: PATRICIA E GALLAGHER
• 金额: $ 13.63万
• 依托单位: WAKE FOREST UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-01 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7063101
• 关键词: RNA interference; antisense nucleic acid; biomedical facility; genetic techniques; molecular cloning; northern blottings; nucleic acid probes; polymerase chain reaction; southern blotting; tissue /cell culture; western blottings

This core will facilitate the use of state-of-the-art cellular and molecular biology technologies in all of the projects in the Program Project. The responsibilities of the core will involve the performance of two separate but related functions: 1) The Tissue Culture unit will provide tissue culture cells and the expertise necessary to complete the cellular biology protocols in the individual proposals and 2) The Molecular Biology unit will provide reagents and expertise to complete the molecular biology experiments described in the five projects. Described below are specific details of the primary methods used in the Core. These include, but are not limited to, reverse transcriptase-polymerase chain reaction, gene array hybridization, Northern, Southern and Western blot analysis, development of antisense oligonucleotides and siRNAs, generation of recombinant adenoviral constructs, subcloning of gene fragments for riboprobe development, and riboprobe synthesis, and promoter analysis. If required, these procedures will be provided to the investigators during the course of the proposed studies and new methodologies will be developed as needed.

该核心将促进在该计划项目的所有项目中使用最先进的细胞和分子生物学技术。核心的职责将涉及执行两个独立但相关的功能：1）组织培养单元将提供组织培养细胞以及完成各个提案中的细胞生物学方案所需的专业知识，2）分子生物学单元将提供完成五个项目中描述的分子生物学实验的试剂和专业知识。下面描述的是 Core 中使用的主要方法的具体细节。这些包括但不限于 逆转录酶-聚合酶链式反应、基因阵列杂交、Northern、Southern 和 Western 印迹分析、反义寡核苷酸和 siRNA 的开发、重组腺病毒构建体的生成、用于核糖核酸探针开发的基因片段亚克隆、核糖核酸探针合成和启动子分析。如果需要，这些程序将在拟议的研究过程中提供给研究人员，并根据需要开发新的方法。