CORE--ANTIOXIDANT ENZYME

核心--抗氧化酶

• 批准号: 6616896
• 负责人: LARRY OBERLEY
• 金额: $ 11.34万
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-07-24 至 2003-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6616896
• 关键词: antisense nucleic acid; biomedical facility; catalase; cell line; complementary DNA; enzyme activity; glutathione peroxidase; myeloperoxidase; superoxide dismutase; transfection /expression vector

DESCRIPTION (provided by applicant): The purpose of the Antioxidant Enzyme Core (AEC) is to provide antioxidant enzyme expertise and analysis for the individual research projects. Discussion between a project investigator and the AEC Director will be held to determine which experiments and/or assays are run. The AEC staff will carry out the proposed work or will advise the investigator on how to perform the experiments. Some of this work, particularly transfections of new cDNAs or in new cell lines as well as the development of antisense reagents, will be developmental in nature. The AEC will aid in enzyme activity assays, western, northern, and Southern analysis. The enzyme assays will be run on the antioxidant enzymes copper-and zinc-containing superoxide dismutase (CuZnSOD), manganese-containing superoxide dismutase (MnSOD), catalase (CAT), and glutathione peroxidase (GPx, both cytosolic and phospholipid). For Project 1, work will also be done on the white cell enzyme myeloperoxidase (MPO). For reagents, we have antibodies and cDNA probes to all primary antioxidant enzymes. In addition, the AEC will aid in transfecting the cDNAs for these enzymes into cell lines of interest to the project investigators. We have experience with several methods of transfection, as well as many different expression vectors for these enzymes. In the course of this work, the AEC will continue to maintain and develop reagents i.e., antibodies, cDNAs, and expression vectors for the antioxidant proteins. The AEC will also have as a goal the development of antisense reagents to inhibit the levels of all of the major antioxidant proteins.

描述（由申请人提供）：抗氧化剂酶的目的 核心（AEC）是为抗氧化剂酶专业知识和分析 个人研究项目。 项目调查员与 AEC主任将被持续以确定哪些实验和/或测定是 跑步。 AEC工作人员将执行拟议的工作，或者建议 研究人员如何执行实验。 其中一些工作， 特别是新cDNA或新细胞系的转染 反义试剂的开发本质上将是发展的。 AEC将有助于酶活性测定，西部，北部和南部 分析。 该酶测定将在抗氧化剂铜上进行铜和 含锌的超氧化物歧化酶（Cuznsod），含锰 超氧化物歧化酶（MNSOD），过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPX， 胞质和磷脂）。 对于项目1，还将完成工作 白细胞酶髓过氧化物酶（MPO）。 对于试剂，我们有抗体 和cDNA探针对所有原代抗氧化剂酶。 此外，AEC将 有助于将这些酶的cDNA转染到感兴趣的细胞系中 项目调查人员。 我们有几种方法的经验 这些酶的转染以及许多不同的表达向量。 在这项工作的过程中，AEC将继续维护和发展 试剂，即抗氧化剂的抗体，cDNA和表达载体 蛋白质。 AEC也将作为反义发展的目标 抑制所有主要抗氧化剂蛋白水平的试剂。