Core--Vector

核心--向量

• 批准号: 6809071
• 负责人: ROBERT M. SAPOLSKY
• 金额: $ 20.94万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-01 至 2009-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6809071
• 关键词: Adenoviridae; Alphaherpesvirinae; BCL2 gene /protein; Retroviridae; antioxidants; biomedical facility; biotechnology; catalase; gene expression; glutathione peroxidase; reporter genes; superoxide dismutase; transfection /expression vector

The vector core will produce herpes simplex virus (HSV)-based amplicon and retroviral vectors to be used in Projects by Giffard and Steinberg. Vectors will be produced expressing the genes for CuZn-superoxide dismutase (SOD1), manganese SOD (SOD2), glutathione peroxidase (GPX), combinations of the antioxidant genes, p35 and crmA and bcl-2. All the vectors will be "bicistronic," expressing both the gene under investigation and a reporter gene. For each experimental vector, the cognate control vector will contain the gene in question with a stop codon inserted in its center, insuring non-expression. Retroviral vectors to express SOD1, SOD2, catalase, GPx and combinations of the antioxidant genes will be produced. Control vectors will express either the reporter gene beta-galactosidase or a stop codon version of the gene of interest. The continuing purposes of the Vector Core are to ensure a constant supply of vectors for the research groups, insure quality control in the production of the vectors, and to troubleshoot problems encountered in the use of the vectors.

矢量核心将产生基于Giffard和Steinberg的项目中使用的基于基于HSV的扩增子和逆转录病毒载体。 将产生载体，以表达氧化甲氧化酶歧化酶（SOD1），锰SOD（SOD2），谷胱甘肽过氧化物酶（GPX），抗氧化基因的组合，p35和crma和bcl-2的组合。 所有矢量都将是“双性恋”，既表示正在研究的基因，又表达记者基因。 对于每个实验矢量，同源控制载体将包含所讨论的基因，并在其中心插入的终止密码子，确保非表达。 将产生逆转录病毒载体，以表达SOD1，SOD2，过氧化氢酶，GPX和抗氧化基因的组合。 控制载体将表达记者基因β-半乳糖苷酶或感兴趣基因的终止密码子版本。 向量核心的持续目的是确保为研究小组提供持续的向量供应，确保矢量生产中的质量控制以及在使用向量时遇到的问题。