CORE--FLOW CYTOMETRY FACILITY

核心——流式细胞仪

• 批准号: 6344761
• 负责人: PETER S RABINOVITCH
• 金额: $ 10.06万
• 依托单位: FRED HUTCHINSON CANCER RESEARCH CENTER
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-06-01 至 2001-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6344761
• 关键词: analytical chemistry; biomedical equipment resource; biomedical facility; catalase; flow cytometry; glutathione; glutathione peroxidase; glutathione reductase; glutathione transferase; high performance liquid chromatography; spectrometry; superoxide dismutase; analytical chemistry; biomedical equipment resource; biomedical facility; catalase; flow cytometry; glutathione; glutathione peroxidase; glutathione reductase; glutathione transferase; high performance liquid chromatography; spectrometry; superoxide dismutase

The Specific Aim of this Core is to provide two categories of services to the Program Project: 1) flow cytometry and cell sorting methods and technologies, and 2) assays of glutathione and the activity of redox enzymes glutathione-s-transferase, superoxide dismutase (S.O.D.) and catalase. We will also assist investigators in finding the most appropriate ways to apply these techniques to their research objectives and to interpret the experimental results. Cytometry and cell sorting will be essential parts of each of the program projects, providing variety of analytical services and techniques. Assays of DNA content, cell cycle and cell proliferation, a novel cytometric assay of oxidative DNA damage and repair, and cytometric assays related to resistance to oxidative damage (glutathione content, cell viability and apoptosis) will constitute major uses of cytometry in this Program Project proposal. In addition, this core will provide non-cytometric assays that will be widely used in the Program Project: HPLC and spectrophotometric quantitative assays of the important intracellular antioxidant glutathione; assays of glutathione-s- transferase, a principal enzyme responsible for carcinogen detoxification; assays of GSH peroxidase and GSH reductase, important enzymes in the synthesis of glutathione, and assays of the oxygen radial scavenging enzymes superoxide dismutase (S.O.D.) and catalase.

该核心的具体目的是为 程序项目：1）流式细胞仪和细胞分类方法以及 技术和2）测定谷胱甘肽和氧化还原活性 酶谷胱甘肽-S-转移酶，超氧化物歧化酶（S.O.D.）和 过氧化氢酶。我们还将协助调查人员找到最多的 适当的方法将这些技术应用于其研究目标 并解释实验结果。细胞仪和细胞分类将 是每个计划项目的重要部分，提供多种多样 分析服务和技术。 DNA含量，细胞周期和 细胞增殖，这是一种新型的氧化DNA损伤的细胞计量测定和 维修和与氧化损伤的抗性有关 （谷胱甘肽含量，细胞活力和凋亡）将构成主要 在此计划项目建议中使用细胞仪。此外，这个核心 将提供将在程序中广泛使用的非周期测定 项目：重要的HPLC和分光光度定量测定 细胞内抗氧化剂谷胱甘肽；谷胱甘肽-S-的测定 转移酶，负责致癌解毒的主要酶； GSH过氧化物酶和GSH还原酶的测定，重要酶 谷胱甘肽的合成和氧气径向清除的测定 酶超氧化物歧化酶（S.O.D.）和过氧化氢酶。