Core--Cellular and Molecular Biology

核心--细胞与分子生物学

• 批准号: 6853124
• 负责人: PATRICIA E GALLAGHER
• 金额: $ 13.23万
• 依托单位: WAKE FOREST UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-01 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6853124
• 关键词: RNA interference; antisense nucleic acid; biomedical facility; genetic techniques; molecular cloning; northern blottings; nucleic acid probes; polymerase chain reaction; southern blotting; tissue /cell culture; western blottings

This core will facilitate the use of state-of-the-art cellular and molecular biology technologies in all of the projects in the Program Project. The responsibilities of the core will involve the performance of two separate but related functions: 1) The Tissue Culture unit will provide tissue culture cells and the expertise necessary to complete the cellular biology protocols in the individual proposals and 2) The Molecular Biology unit will provide reagents and expertise to complete the molecular biology experiments described in the five projects. Described below are specific details of the primary methods used in the Core. These include, but are not limited to, reverse transcriptase-polymerase chain reaction, gene array hybridization, Northern, Southern and Western blot analysis, development of antisense oligonucleotides and siRNAs, generation of recombinant adenoviral constructs, subcloning of gene fragments for riboprobe development, and riboprobe synthesis, and promoter analysis. If required, these procedures will be provided to the investigators during the course of the proposed studies and new methodologies will be developed as needed.

该核心将有助于在程序项目中的所有项目中使用最先进的细胞和分子生物学技术。核心的责任将涉及两个单独但相关的功能的表现：1）组织培养单元将提供组织培养细胞，并提供完成单个建议中细胞生物学方案的必要专业知识，2）分子生物学单元将提供试剂和专业知识，以完成五个项目中描述的分子生物学实验。下面描述的是核心中使用的主要方法的具体细节。这些包括但不限 逆转录酶 - 聚合酶链反应，基因阵列杂交，北部，南部和蛋白质印迹分析，反义寡核苷酸和siRNA的开发，重组腺病毒构建体的产生，基因片段的亚克隆，用于核糖核苷的发育以及核糖核苷的合成和促进剂分析。如果需要，将在拟议的研究过程中向研究人员提供这些程序，并根据需要开发新的方法。