Optical Imaging of S1 Cortex in the Awake Primate

清醒灵长类动物 S1 皮层的光学成像

• 批准号: 7105403
• 负责人: ANNA W. ROE
• 金额: $ 20.62万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2008-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7105403
• 关键词: Primates; attention; dyes; electrical potential; touch; wakefulness

DESCRIPTION (provided by applicant): The goal of this research proposal is to develop the optical imaging methodology for use in awake, behaving monkeys. Optical imaging has been highly successful in revealing cortical organization in anesthetized animals. We propose to develop this method to study cortical activation patterns in somatosensory physiological responses of somatosensory cortex in anesthetized an awake monkeys. However, little is known about the spatial patterns of activation in awake, behaving animals. The proposed methodology is a highly novel approach to the study of somatosensory cortical function. By directly relating high spatial and temporal resolution images of cortical activation patterns to behavior, it promises to have a high impact on views of cortical function. We have two aims. One aim is to develop an optical imaging system capable of both high spatial and temporal resolution for use in awake, behaving monkeys. Both intrinsic signal and voltage sensitive dye imaging capabilities will be incorporated into a single system. This involves hardware and software development, testing in behaving monkeys, and development of new data analysis methods. Our second aim is to do exploratory optical imaging studies in awake monkeys performing tactile tasks. There are significant differences in topography, function organization, and inter-areal activations between anesthetized an awake SI. We aim to explore whether these differences correlate with factors such as attention and task- dependent context. Given our preliminary data, it is highly likely that these studies will prove fruitful and will form the basis for future R01s in behaving monkeys. The proposed methodology is novel and will permit a new approach to the study of cognitive functions. Further, this type of functional imaging in behaving monkeys will provide a much needed link between bodies of single-unit physiology, fMRI, and behavioral studies.

描述（由申请人提供）：本研究计划的目标是开发用于清醒、行为正常的猴子的光学成像方法。光学成像在揭示麻醉动物的皮质组织方面非常成功。我们建议开发这种方法来研究麻醉清醒猴子体感皮层体感生理反应的皮层激活模式。然而，人们对清醒、有行为的动物的激活空间模式知之甚少。所提出的方法是研究体感皮质功能的一种非常新颖的方法。通过直接将皮质激活模式的高空间和时间分辨率图像与行为联系起来，它有望对皮质功能的观点产生重大影响。我们有两个目标。一个目标是开发一种具有高空间和时间分辨率的光学成像系统，用于清醒、行为正常的猴子。固有信号和电压敏感染料成像功能将被整合到一个系统中。这涉及硬件和软件开发、行为猴子测试以及新数据分析方法的开发。我们的第二个目标是对执行触觉任务的清醒猴子进行探索性光学成像研究。麻醉和清醒 SI 之间的地形、功能组织和区域间激活存在显着差异。我们的目的是探讨这些差异是否与注意力和任务相关背景等因素相关。根据我们的初步数据，这些研究很可能会取得丰硕成果，并为未来猴子行为 R01 奠定基础。所提出的方法是新颖的，将为认知功能的研究提供新的方法。此外，这种对行为猴子进行的功能成像将为单单位生理学、功能磁共振成像和行为研究之间提供急需的联系。