Fast Optical Imaging of Cortical Signals in the Behaving Primate

行为灵长类动物皮层信号的快速光学成像

• 批准号: 7139774
• 负责人: ANNA W. ROE
• 金额: $ 22.96万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-30 至 2008-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7139774
• 关键词: Macaca; Primates; behavior; cognition; dyes; electrical potential; fasting; prefrontal lobe /cortex; short term memory; wakefulness

DESCRIPTION (provided by applicant): The goal of this research proposal is to develop a fast temporal resolution optical imaging methodology for use in awake, behaving monkeys. If successful, this methodology would provide online visualization of brain function at both high spatial (10's of ums) and high temporal (1-10 msec) resolution. Intense effort has been devoted to studying anatomical organization and physiological responses of sensory cortex in anesthetized and awake monkeys. However, little is known about the spatial patterns of activation in prefrontal cortex of awake, behaving animals during performance of working memory tasks. The goal is to observe clustered and distributed clusters of neuronal activation of cortical activity during behavior. These data would also provide a critical link between single-unit physiology, functional imaging studies, and behavioral studies. Importantly, the proposed technology development will provide new methodologies for studying spatial (functional organizational) and temporal aspects of cortical processing relating to vision, attention, memory, and cognitive function. Clinical relevance of data obtained from this approach would be relevant to cognitive dysfunctions in diseases such as schizophrenia. The specific aims of this proposal are: 1) development of fast optical imaging system for awake, behaving monkeys. This aim would include development of voltage sensitive dye methodology, noise reduction techniques for optical detection of neuronal activity, rapid image processing techniques suitable for large volumes of streamed data, and tandem electrophysiological recordings. 2) application of this method to study working memory processes in prefrontal cortex. Macaque monkeys would be trained on oculomotor delay response tasks. Delay period activity in prefrontal cortex would be imaged at high temporal (1-10 msec) to monitor changing spatiotemporal activity patterns depending on task demands. This methodology will provide a new approach for examining relationship of cortical organization with behavior, one that will complement the spatiotemporal capabilities of other existing technologies such as fMRI and single unit electrophysiology.

描述（由申请人提供）： 该研究建议的目的是开发快速的时间分辨率光学成像方法，用于醒来，表现猴子。如果成功的话，这种方法将在高空间（UMS 10）和高时间（1-10毫秒）分辨率上提供大脑功能的在线可视化。激烈的努力致力于研究麻醉和清醒猴子中感觉皮层的解剖组织和生理反应。然而，关于清醒前额叶皮层中激活的空间模式，在工作记忆任务执行过程中表现不佳。目的是观察行为过程中皮质活性神经元激活的聚类和分布的簇。这些数据还将在单单体生理学，功能成像研究和行为研究之间提供关键的联系。重要的是，拟议的技术开发将为研究空间（功能组织）和与视觉，注意力，记忆和认知功能有关的皮质处理的时间方面提供新的方法。从这种方法获得的数据的临床相关性与精神分裂症等疾病的认知功能障碍有关。该提案的具体目的是：1）开发快速光学成像系统以醒来，行为猴子。该目的将包括开发电压敏感染料方法，降噪技术，用于光学检测神经元活性，快速图像处理技术适合大量流媒体数据，以及串联电生理记录。 2）应用这种方法在前额叶皮层中研究工作记忆过程。猕猴将接受有关动眼延迟响应任务的培训。前额叶皮层中的延迟周期活动将在高时间（1-10毫秒）上成像，以根据任务需求监测变化的时空活动模式。该方法将为研究皮质组织与行为的关系提供一种新的方法，该方法将补充其他现有技术（例如fMRI和单个单位电生理学）的时空能力。