Regulation of Polarised Trafficking Machinery in Epithelial to Mesenchymal Transition.

上皮间质转化中极化运输机制的调节。

• 批准号: 2609149
• 金额: --
• 依托单位: University of Dundee
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2021
• 资助国家: 英国
• 起止时间: 2021 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2609149
• 关键词: Regulation; Polarised; Trafficking; Machinery; Epithelial

During epithelial to mesenchymal transition (EMT), epithelial cells lose their apical-basal polarity and gain invasive properties. Currently, it is unknown how the regulation of trafficking machinery which mediates polarised trafficking is altered. Included in the trafficking machinery is the exocyst complex. One of the key hallmarks of cancer is the activation of invasion and metastasis, this directly correlates with a poor prognosis in patients. The ability of cells to undergo EMT is central to metastasis. It is therefore essential to better understand changes to polarised trafficking which facilitate this step. To address these questions, quantitative mass spectrometry will be used to identify exocyst interacting proteins during EMT. This research willprovide an insight into one of the major challenges in modern healthcare. Moreover, cell biology and recombinant protein biochemistry methods will be used to further characterise exocyst dynamics at the basal membrane and the role of exocyst interactors

在上皮间质转化（EMT）过程中，上皮细胞失去顶端-基底极性并获得侵袭特性。目前，尚不清楚调解两极分化贩运的贩运机制的监管如何改变。贩运机制中包括外囊复合体。癌症的关键标志之一是侵袭和转移的激活，这与患者的不良预后直接相关。细胞进行 EMT 的能力对于转移至关重要。因此，有必要更好地了解促进这一步骤的两极分化贩运的变化。为了解决这些问题，定量质谱法将用于鉴定 EMT 过程中的胞外相互作用蛋白。这项研究将深入了解现代医疗保健的主要挑战之一。此外，细胞生物学和重组蛋白生物化学方法将用于进一步表征基底膜的外囊动力学以及外囊相互作用因子的作用