Single Fibril Mechanics

单原纤维力学

• 批准号: 7140188
• 负责人: Steven J Eppell
• 金额: $ 18.33万
• 依托单位: CASE WESTERN RESERVE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-15 至 2009-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7140188
• 关键词: Echinodermata; atomic force microscopy; bioengineering /biomedical engineering; bioimaging /biomedical imaging; biomechanics; biomedical equipment development; bone fracture; collagen; confocal scanning microscopy; disease /disorder proneness /risk; evaluation /testing; mechanical stress; miniature biomedical equipment; musculoskeletal disorder diagnosis; musculoskeletal imaging /visualization /scanning; nanotechnology; tensile strength; tissue /cell preparation

DESCRIPTION (provided by applicant): This exploratory R21 proposal is to build a novel microelectromechanical system (MEMS) device for direct measurement of the mechanical properties of a collagen fibril. Usually these properties are inferred from larger scale measurements' yielding model dependent results and making it difficult to sort out nanoscale from higher level structure-function relationships. The proposed device is a modification of an existing MEMS device originally designed to test silicon materials at the microscale. We have modified the design of the device for use with smaller specimens having lower moduli. The device fills in a gap between the piconewton level techniques used to measure single molecules and the millinewton devices commercially available for the study of micron to millimeter sized samples. Our group's use of the machine will be primarily to test collagen fibrils relevant to bone. However, we expect the device will have wide applicability in the study of extracellular matrix fibrils in tissues ranging from tendon and ligament to cartilage and bone. Specific Aims 1. Design and fabricate a Microelectromechanical System (MEMS) useful for testing nanofibrils 2. Develop and implement protocols for attaching nanofibrils to MEMS device 3. Test device and protocols using collagen fibrils.

描述（由申请人提供）： 这项探索性 R21 提案旨在构建一种新型微机电系统 (MEMS) 设备，用于直接测量胶原纤维的机械性能。通常这些特性是从更大规模的测量中推断出来的，产生依赖于模型的结果，并且使得很难从更高层次的结构-功能关系中找出纳米尺度。所提出的设备是对现有 MEMS 设备的改进，最初设计用于在微尺度上测试硅材料。我们修改了设备的设计，以用于具有较低模量的较小样本。该设备填补了用于测量单分子的皮牛顿级技术与用于研究微米至毫米尺寸样品的市售毫牛顿设备之间的空白。我们小组使用该机器将主要用于测试与骨骼相关的胶原纤维。然而，我们预计该设备将在肌腱、韧带、软骨和骨骼等组织中的细胞外基质原纤维的研究中具有广泛的适用性。具体目标 1. 设计和制造可用于测试纳米纤丝的微机电系统 (MEMS) 2. 开发并实施将纳米原纤维附着到 MEMS 器件的协议 3. 使用胶原纤维的测试装置和方案。