Protein Engineering and Biosensing Interface Materials

蛋白质工程和生物传感界面材料

基本信息

批准号：
7120164
负责人：
EDWARD ETESHOLA
金额：
$ 14.19万
依托单位：
OHIO STATE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2005
资助国家：
美国
起止时间：
2005-09-06 至 2010-08-31
项目状态：
已结题

项目摘要

DESCRIPTION (provided by candidate): Candidate: Dr. Edward Eteshola's long term career goal is to become an independent investigator in advanced biomaterials and biosensor development research for biomedical and biotechnological applications. His immediate objective through this Career Development Award proposal is to acquire new training and skills in advanced modern protein engineering methodologies to generate novel engineered biomaterials for biosensor surface and interface design to enable this goal. He has designed a career development plan that incorporates modern advances in biomolecular engineering to prepare novel interfacial biomaterials for the rational design and fabrication of functional affinity sensor surfaces/interfaces. Environment: The rich research environment at the Ohio State University, Columbus is ideal for the candidate's career development; there are several successful units with extramurally funded researchers in the areas of protein engineering, immunology, biochemistry, surface and interface science. Dr. Eteshola will be mentored by Drs. Lee and Brillson. Drs. Lee and Brillson are expert protein engineer and materials surface scientist, respectively. Their combined labs will provide excellent environments for developing the skills necessary to successfully execute the details of this proposal. Research: Biochemically modified field-effect transistor (BioFET) based systems are intended to detect and quantitate charge carrying biomolecules (e.g., proteins) in a label-free manner, i.e. without need for reporter molecules. Although, these affinity BioFET sensors have reportedly been used to detect DNA hybridization reactions, detection of proteins by this method has yet to be achieved on a practical level. A main reason for this difficulty is that the large size of the surface bound receptor proteins prevents the target ligands from approaching FET sensing channel sufficiently closely for efficient detection of change in surface electrical charge properties following receptor-ligand binding event occurring on the sensor device. This application proposes a novel approach for the bio/engineering and fabrication of FET sensor surfaces/interfaces at the molecular level by engineering novel interfacial biomolecules with desirable biochemical and physical characteristics (small size, selectivity, novel binding properties) specifically adapted for use in FET sensor device environment; this approach is expected to greatly improve interfacial interaction phenomena, and thus improved signal transduction, resulting in the realization of more specific, sensitive and stable BioFET sensor devices. The success of this project could have significant impact not only in realizing novel biosensor technologies but as well in the broader fields of MEMS micro- and nano-devices, bioengineered coatings for implantable devices, tissue engineering scaffolds, etc. where surface/interface phenomena are critically important.

描述（由候选人提供）：候选人：爱德华·埃特索拉（Edward Eteshola）博士的长期职业目标是成为生物医学和生物技术应用的高级生物材料和生物传感器开发研究的独立研究者。他通过该职业发展奖提案的直接目标是获得先进的现代蛋白质工程方法的新培训和技能，以生成用于生物传感器表面和界面设计的新型工程生物材料，以实现这一目标。他设计了一项职业发展计划，该计划结合了生物分子工程的现代进步，以准备新型的界面生物材料，以实现功能亲和力传感器表面/界面的理性设计和制造。环境：俄亥俄州立大学的丰富研究环境，哥伦布是候选人职业发展的理想选择；在蛋白质工程，免疫学，生物化学，表面和界面科学领域，有几个成功的单元，具有外部资助的研究人员。 Eteshola博士将由Drs指导。李和布里尔逊。博士。 Lee和Brillson分别是专家蛋白质工程师和材料表面科学家。他们的合并实验室将为开发成功执行该建议细节所需的技能提供良好的环境。研究：基于生物化学修饰的现场效应晶体管（Biofet）系统旨在以无标记的方式检测和定量携带携带的生物分子（例如蛋白质），即无需记者分子。尽管据报道，这些亲和力生物源传感器已用于检测DNA杂交反应，但通过这种方法检测蛋白质尚未在实际水平上实现。造成这种困难的主要原因是，大尺寸的表面结合受体蛋白可防止靶配体足够接近FET感测通道，以有效检测在传感器设备上发生受体配体结合事件后表面电荷特性的变化。该应用提出了一种新的方法，用于通过工程新型的生物化学和物理特征（小尺寸，选择性，新型结合特性），专门适用于fet传感器设备环境中，使用具有理想的生化和物理特征（小型，选择性，新型结合特性）的新型界面生物分子，用于分子水平的FET传感器表面/界面的新方法。预计这种方法将大大改善界面相互作用现象，从而改善信号转导，从而导致实现更具体，敏感和稳定的生物反应传感器设备。该项目的成功不仅在实现新型生物传感器技术方面，而且在MEMS微型和纳米设备的更广泛领域，可植入设备的生物工程涂层，组织工程支架等。