BIOMIMETIC SCAFFOLD FOR BONE-REPAIR

用于骨修复的仿生支架

• 批准号: 6815689
• 负责人: Anne George
• 金额: $ 31.84万
• 依托单位: UNIVERSITY OF ILLINOIS AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-07-01 至 2008-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6815689
• 关键词: atomic force microscopy; bioengineering /biomedical engineering; biomaterial compatibility; biomaterial development /preparation; biomimetics; cell adhesion; cell differentiation; circular dichroism; electron microscopy; extracellular matrix; implant; intermolecular interaction; laboratory rat; medical implant science; molecular assembly /self assembly; musculoskeletal disorder therapy; musculoskeletal injury; normal ossification; osteoblasts; osteogenesis; tissue engineering; tissue support frame

DESCRIPTION (provided by applicant): Biomineralization processes such as formation of bones and teeth require controlled mineral deposition and self-assembly into hierarchical biocomposites with unique mechanical properties. Ideal biomaterials for regeneration and repair of intramembranous and endochondral bone must be biocompatible, an optimal template for vascular invasion, facilitate normal differentiation of osteoprogenitor cells, ideal for deposition of mineral and possess intrinsic biocompatible properties. Novel methods of designing tissue repair and restoration materials using bioinspired strategies are currently in vogue. Therefore, it is possible that hierarchically organized nanomaterials can be constructed by tailor-made peptide sequences with self-assembling properties. Our overall goal is to employ novel strategies for fabrication of biomaterial for bone repair with a three-dimensional microstructure, by incorporating peptides with self-assembly characteristics along with spatial presentation of bioactive ligands that would provide a physiological environment for cells, facilitating proliferation, differentiation and inducing mineralized tissue formation. Specifically we will: (1) Design and fabricate self-assembled matrices that contain motifs for self-assembly, cell-adhesion and mineral induction; (2) Test the functionality's of the motifs incorporated in the scaffold (3) Test the osteogenic activity of the scaffold in vivo.

描述（由申请人提供）：生物矿化过程，例如骨骼和牙齿的形成，需要受控的矿物质沉积和自组装成具有独特机械性能的分层生物复合材料。用于膜内骨和软骨内骨再生和修复的理想生物材料必须具有生物相容性，是血管侵袭的最佳模板，促进骨祖细胞的正常分化，是矿物质沉积的理想选择，并具有内在的生物相容性。使用仿生策略设计组织修复和修复材料的新方法目前很流行。因此，可以通过具有自组装特性的定制肽序列来构建分层组织的纳米材料。我们的总体目标是采用新颖的策略来制造具有三维微结构的骨修复生物材料，通过结合具有自组装特性的肽以及生物活性配体的空间呈现，为细胞提供生理环境，促进增殖、分化并诱导矿化组织形成。 具体来说，我们将： (1) 设计和制造包含自组装、细胞粘附和矿物质诱导图案的自组装基质； (2) 测试支架中纳入的基序的功能 (3)测试支架的体内成骨活性。