Single Fibril Mechanics

单原纤维力学

• 批准号: 7042998
• 负责人: Steven J Eppell
• 金额: $ 22.36万
• 依托单位: CASE WESTERN RESERVE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-15 至 2007-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7042998
• 关键词: Echinodermata; atomic force microscopy; bioengineering /biomedical engineering; bioimaging /biomedical imaging; biomechanics; biomedical equipment development; bone fracture; collagen; confocal scanning microscopy; disease /disorder proneness /risk; evaluation /testing; mechanical stress; miniature biomedical equipment; musculoskeletal disorder diagnosis; musculoskeletal imaging /visualization /scanning; nanotechnology; tensile strength; tissue /cell preparation

DESCRIPTION (provided by applicant): This exploratory R21 proposal is to build a novel microelectromechanical system (MEMS) device for direct measurement of the mechanical properties of a collagen fibril. Usually these properties are inferred from larger scale measurements' yielding model dependent results and making it difficult to sort out nanoscale from higher level structure-function relationships. The proposed device is a modification of an existing MEMS device originally designed to test silicon materials at the microscale. We have modified the design of the device for use with smaller specimens having lower moduli. The device fills in a gap between the piconewton level techniques used to measure single molecules and the millinewton devices commercially available for the study of micron to millimeter sized samples. Our group's use of the machine will be primarily to test collagen fibrils relevant to bone. However, we expect the device will have wide applicability in the study of extracellular matrix fibrils in tissues ranging from tendon and ligament to cartilage and bone. Specific Aims 1. Design and fabricate a Microelectromechanical System (MEMS) useful for testing nanofibrils 2. Develop and implement protocols for attaching nanofibrils to MEMS device 3. Test device and protocols using collagen fibrils.

描述（由申请人提供）： 该探索性R21建议是建立一种新型的微电力系统（MEMS）设备，以直接测量胶原原纤维的机械性能。通常，这些属性是从大规模测量的产生模型依赖性结果中推断出来的，并且很难从更高级别的结构 - 功能关系中分析纳米级。所提出的设备是对最初旨在测试微观材料的现有MEMS设备的修改。我们已经修改了该设备的设计，以与具有较低模量的较小样品一起使用。该设备填补了用于测量单分子的Piconewton水平技术和商业上可用于研究微米至毫米大小的样品的千分设备的差距。我们小组对机器的使用将主要用于测试与骨骼相关的胶原纤维。但是，我们预计该设备将在研究肌腱和韧带到软骨和骨骼等细胞外基质原纤维的研究中具有广泛的适用性。具体目标 1。设计和制造微电机电系统（MEMS），可用于测试纳米纤维 2。开发并实施将纳米纤维连接到MEMS设备的协议 3。使用胶原纤维的测试装置和协议。