Cooperative Human Tissue Network

合作人体组织网络

• 批准号: 7233397
• 负责人: CARL D MORRISON
• 金额: $ 86.64万
• 依托单位: OHIO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-06-01 至 2007-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7233397
• 关键词: biomedical registry /referral center; cancer information system; cancer registry /resource; case history; computer system design /evaluation; cooperative study; histopathology; human tissue; nucleic acids; tissue resource /registry

The objective of this proposal is to continue to operate a highly productive, efficient tissue procurement service at The Ohio State University Medical Center as a part of the Cooperative Human Tissue Network (CHTN) of the National Cancer Institute (NCI). This division will manage procurement consortiums both in Central (OSU Hospitals, James Cancer Hospital and Research Institute, and Riverside Methodist Hospital) and Northern Ohio (Cleveland Clinic) for research investigators in the United States and Canada. As the Midwestern Division of the CHTN we will continue to provide human normal, diseased, benign, and malignant surgical and autopsy tissues. This includes solid tissue, blood/sera, ascites fluids and pheresis specimens. Remnant tissues obtained for the CHTN will be examined and selected grossly then further quality controlled microscopically with histological review by a surgical pathologist. Tissues will be procured by investigator-specified guidelines and shipped within 24 hours or in batches according to the investigator's needs. Typical methods of procurement include liquid nitrogen (LN2) snap frozen tissues, OCT medium frozen tissue in LN2 cooled isopentane, gauze-wrapped fresh tissues in cooled saline, fresh tissue in transport tissue culture mediums and supplements, and formalin or gluteraldehyde fixed tissues. Paraffin- embedded fixed tissues are also available. In this grant new alternative methods for processed tissue will include RNase-inhibited prepared tissue, extracted tumor DNA and RNA, and slides of tumor sets in paraffin-embedded tissue matrix arrays. Investigator applications will be prioritized according to the CHTN charter. This division will continue to work closely with the other CHTN divisions to network investigator requests nationally, to educate the research community on the availability of CHTN specimens, to actively participate in the CHTN agendas to improve services and develop CHTN-wide policy and protocols. OSU Medical Center is instituting a system-wide informed consent for patients that will allow the use of specimens for research with outcome data or specimens will be delinked for complete anonymity. Specimens will continue to be coded to ensure patient confidentiality. OSU CHTN employees consist of anatomic technologists fully trained and supervised by pathologists in techniques of gross tissue examination, office personnel who perform database entry, update investigator's requests, distribute pathology reports, report statistical data, and gather donor-related medical information. In addition, the OSU CHTN personnel include a pathologist in charge of the daily operations.

该提案的目的是继续在俄亥俄州立大学医学中心运营高产，高效的组织采购服务，作为国家癌症研究所（NCI）合作人类组织网络（CHTN）的一部分。该部门将管理中央（OSU医院，詹姆斯癌症医院和研究所，河滨卫理公会医院）和俄亥俄州北部（克利夫兰诊所）的采购财团，用于美国和加拿大的研究研究人员。作为CHTN的中西部分裂，我们将继续提供人类的正常，患病，良性和恶性外科手术和尸检组织。这包括固体组织，血液/血清，腹水液和体面样本。将检查和选择CHTN获得的残留组织，然后进一步控制质量，并通过手术病理学家进行组织学评论。组织将由研究人员指定的指南采购，并在24小时内或根据研究人员的需求进行批量运行。典型的采购方法包括液氮（LN2）SNAP冷冻组织，在LN2冷却的异戊烷中的OCT中等冷冻组织，冷却的盐水中的纱布包裹的新鲜组织，在传输组织培养基中的新鲜组织和补充剂中的新鲜组织，以及福尔托林或戊二醛固定组织。还提供了石蜡嵌入的固定组织。在这项赠款中，用于加工组织的新替代方法将包括抑制RNase的组织，提取的肿瘤DNA和RNA，以及在石蜡包含的组织基质阵列中的肿瘤载玻片。根据CHTN宪章将优先考虑研究者的申请。该部门将继续与其他CHTN部门紧密合作，向网络调查员在全国范围内要求，以教育研究界的CHTN标本的可用性，以积极参与CHTN议程以改善服务并制定CHTN范围内的政策和协议。 OSU医疗中心正在为患者征得全系统知情同意，该同意书将允许使用标本进行研究数据或标本的研究，以完全匿名。标本将继续进行编码，以确保患者机密性。 OSU CHTN员工由解剖技术专家组成，由病理学家进行了全面培训和监督，该技术人员在组织检查技术方面，办公室人员进行数据库输入，更新研究者的要求，分发病理学报告，报告统计数据并收集与捐助者相关的医疗信息。此外，OSU CHTN人员还包括负责日常操作的病理学家。