Core--Microarray Facility

核心——微阵列设备

• 批准号: 6990228
• 负责人: Stephen M Prescott
• 金额: $ 2.93万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-07-09 至 2006-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6990228
• 关键词: biomedical facility; genetic library; genetic registry /resource /referral center; microarray technology; molecular biology information system; neoplasm /cancer

The Microarray Shared Resource provides microarray services to members of the Cancer Center as well as to other University investigators. These services include printing of microarray slides, analysis of samples on an Agilent BioAnalyzer to define RNA quality, labeling of samples with Cy3 and Cy5 fluorescent dyes, microarray hybridization, microarray scanning and data capture, data analysis, and cDNA clone distribution. The facility has acquired several key pieces of instrumentation including an Amersham Lucidea Array Spotter, an Axon 4000B Scanner, an Agilent BioAnalyzer, two BioMicro Systems MAUI hybridization units, a Packard Instruments Multiprobe II liquid handler, and an MJ Research Tetrad thermal cycler. Scanned arrays are quantified using BioDiscovery ImaGene software; GeneSight software is available to clients of the Microarray Resource for gene mining and data analysis of their arrays. A microarray laboratory information management system is used to track clones, slides, workflow, samples, experiment requests and data. The Microarray Resource has acquired an extensive collection of cDNA clones that includes human (42,000), mouse (34,000), yeast ORFs (6,000), yeast intergenic sequences (6,000), Drosophila (12,000), Planaria (4,000) and newt (1,500). On an annual basis, approximately 2000 microarray analyses are performed. The Microarray Resource proposes to enhance services in the following areas: expansion of the current clone collections, enhancement of microarray data storage capability, and increased levels of support for microarray data analysis and interpretation. To expand our ability to offer microarrays derived from experimental organisms actively used in cancer studies, we hope to acquire a zebrafish oligo library collection. To improve data storage capabilities of the Microarray Resource, we plan to acquire an Iomega NAS P850m data storage server (funding requested in the Informatics Shared Resource). An additional area of emphasis at the facility will be to provide consultation in experimental design, analysis and interpretation of data. To accomplish this goal, we have added a staff member to focus on consulting with our clients in these areas.

微阵列共享资源为癌症中心以及其他大学调查人员提供了微阵列服务。这些服务包括打印微阵列幻灯片，对安捷伦生物分析仪上的样品进行分析以定义RNA质量，标记具有CY3和CY5荧光染料的样品，微阵列杂交，微阵列扫描和数据捕获，数据分析以及cDNA克隆分布。 该设施已经购买了几种关键的仪表，包括Amersham Lucidea阵列发现者，Axon 4000b扫描仪，Agilent Bioanalyzer，两个BioMicro Systems Maui Maui杂交单元，Packard Instruments Multiprobe II液体处理程序和MJ Research terad Hytarad Thermal Cycler。使用Biodiscovery Imagene软件对扫描阵列进行量化； Microaray资源的客户可用于基因挖掘和阵列的数据分析。微阵列实验室信息管理系统用于跟踪克隆，幻灯片，工作流程，样品，实验请求和数据。微阵列资源已获得广泛的cDNA克隆集合，其中包括人（42,000），鼠标（34,000），酵母ORF（6,000），酵母菌级序列（6,000），果蝇（12,000），Planaria，Planaria （4,000）和Newt（1,500）。每年进行大约2000个微阵列分析。微阵列资源建议在以下领域增强服务：当前克隆集合的扩展，增强微阵列数据存储能力以及对微阵列数据分析和解释的支持水平提高。为了扩大我们提供从癌症研究中积极使用的实验生物的微阵列的能力，我们希望获得斑马鱼寡核心图书馆收藏。为了提高微阵列资源的数据存储功能，我们计划获取Iomega NAS P850M数据存储服务器（信息共享资源中请求的资金）。该设施的另一个重点将是在数据的实验设计，分析和解释方面提供咨询。为了实现这一目标，我们添加了一名工作人员，以专注于在这些领域与客户咨询。