Core--Nuclear Magnetic Resonance Facility

核心——核磁共振装置

• 批准号: 6990230
• 负责人: Stephen M Prescott
• 金额: $ 1.65万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-07-09 至 2006-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6990230
• 关键词: biomedical facility; neoplasm /cancer; nuclear magnetic resonance spectroscopy

The Biomolecular Nuclear Magnetic Resonance (NMR) Shared Resource provides a state-of-the-art laboratory for biomolecular structure determination as well as routine analytical NMR services for the health sciences research community. It currently has three NMR spectrometers operating at 400, 500, and 600 MHz. The 500 MHz spectrometer will be upgraded in 2003 with all new electronics (the "console"). Upon upgrade of the 500 MHz machine, both the 500 and 600 MHz spectrometers will be state-of-the-art for biomolecular NMR, with 3 radiofrequency channels plus 2H decoupling. The 600 MHz spectrometer (Varian Inova) is the highest field NMR spectrometer in the state of Utah. The 400 MHz spectrometer (Varian Mercury) is dedicated to small molecule, organic and natural products NMR applications, and is exceptionally versatile and easy to use. The NMR facility also has several Sun and Silicon Graphics Inc. workstations for offline data processing and biomolecular structure determination. These computers are part of the Structural Biology Computing Center. Software on the SGIs includes VNMR, Insight/Discover, Felix, NMRpipe, XEASY, SPARKY, MOLMOL, CNS, XPLOR and DYANA. The resource is supervised by two faculty directors with established research programs that utilize NMR. In addition, a Ph.D.-level resource manager is responsible for technical support for the most demanding protein and RNA structure determination projects. This manager is responsible for implementing new heteronuclear 3D and 4D NMR experiments for biomolecular NMR, and assisting researchers with data collection as well as data processing and analysis. The resource also has a full-time NMR technician responsible for running small-molecule NMR experiments on the 400 MHz and 500 MHz spectrometers. The NMR technician also trains new users of the NMR spectrometers and performs routine maintenance.

生物分子核磁共振（NMR）共享资源为健康科学研究社区提供了生物分子结构确定以及常规分析NMR服务的最先进实验室。目前，它的三个NMR光谱仪在400、500和600 MHz下运行。 500 MHz光谱仪将于2003年使用所有新电子（“控制台”）升级。升级500 MHz机器后，500和600 MHz光谱仪将是生物分子NMR的最先进，具有3个射频通道加2H解耦。 600 MHz光谱仪（Varian Inova）是犹他州最高的场NMR光谱仪。 400 MHz光谱仪（Varian Mercury）专用于小分子，有机和天然产品NMR应用，并且具有特殊性且易于使用。 NMR设施还拥有几个Sun和Silicon Graphics Inc.离线数据处理和生物分子结构确定的工作站。这些计算机是结构生物学的一部分 计算中心。 SGIS上的软件包括VNMR，Insight/Discover，Felix，Nmrpipe，Xeasy，Sparky，Molmol，CNS，Xplor和Dyana。该资源由两位使用NMR的既定研究计划进行监督。此外，博士级资源经理负责为最苛刻的蛋白质和RNA结构确定项目提供技术支持。该经理负责为生物分子NMR实施新的异核3D和4D NMR实验，并协助研究人员进行数据收集以及数据处理和分析。该资源还拥有一名全日制NMR技术人员，负责在400上运行小分子NMR实验 MHz和500 MHz光谱仪。 NMR技术人员还训练NMR光谱仪的新用户并执行例行维护。