Investigating the utility and exploring biological targets of enhanced micro-wound healing compounds in 2D and 3D skin models

研究增强微伤口愈合化合物在 2D 和 3D 皮肤模型中的效用并探索其生物靶标

• 批准号: 2617469
• 金额: --
• 依托单位: Queen Mary University of London
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2021
• 资助国家: 英国
• 起止时间: 2021 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2617469
• 关键词: Investigating; utility; exploring; biological; targets

Micro-wound healing is a dynamic, physiological process in which keratinocytes transition to an activated, migratory state to re-establish the epidermal barrier and resurface wounds. Although such wounds typically heal, effective intervention could offer enhanced micro-wound healing within mechanistically relevant timeframes. Whilst this is a rapidly evolving area, the successful development of enhanced micro-wound healing compounds (EMH compounds) requires systematic investigation of their utility and exploration of candidate protein targets. An important hurdle is the establishment of a 3D model with highly reproducible micro-wounds and cutting-edge approaches to monitor healing dynamics. We have established 2D and 3D human living skin equivalent (LSEs) models that enable us to perform automated high content image analysis to generate 'multidimensional drug profiles'. In this proposal we integrate our recently established 3D bioprinted models of wound healing to investigate the utility of EMH compounds and explore biological targets of enhanced micro-wound healing.

微链愈合是一个动态的生理过程，在该过程中，角质形成细胞过渡到激活的迁移状态，以重新建立表皮屏障和重新表面伤口。尽管这种伤口通常愈合，但有效的干预措施可以在机械上相关的时间范围内提供增强的微型愈合。尽管这是一个迅速发展的领域，但成功发展了增强的微链愈合化合物（EMH化合物），需要系统地研究其效用和对候选蛋白靶标的探索。一个重要的障碍是建立具有高度可重现的微链路和尖端方法以监测愈合动态的3D模型。我们已经建立了2D和3D人类生活皮肤（LSES）模型，使我们能够执行自动化的高内容图像分析以产生“多维药物概况”。在此提案中，我们整合了最近建立的3D生物打印模型的伤口愈合模型，以研究EMH化合物的效用，并探索增强的微链球愈合的生物学靶标。