BRANCHED CHAIN AMINO ACID BIOSYNTHESIS IN E COLI

大肠杆菌中支链氨基酸的生物合成

• 批准号: 6744706
• 负责人: G. WESLEY HATFIELD
• 金额: $ 29.76万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-05-01 至 2006-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6744706
• 关键词: Escherichia coli; aminoacid biosynthesis; bacterial genetics; branched chain aminoacid; genetic regulation; genetic transcription; microarray technology; microorganism culture; microorganism metabolism; operon

DESCRIPTION: (Adapted from the Investigator's abstract): Our current understanding of gene regulation in Escherichia coli suggests three hierarchical levels of control: 1) global control of basal level gene expression by chromosome structure; 2) global regulatory proteinmediated control of stimulons and regulons; and, 3) operon specific controls. The first level of control is exemplified by the DNA-supercoiling-dependent mechanisms described for the coordination of basal level expression of operons of the ilv regulon. It has been shown that these controls can be influenced by the topological structure of the chromosome, and that DNA architectural proteins such as IHF are able to modulate the formation and location of these structures. The experiments described in this proposal are designed to further characterize this first level of global gene regulation. It is proposed that regulation of gene expression by chromosome structure is influenced by the energy charge of the cell, which in turn is influenced by nutritional and environmental conditions that require transitions from one growth state to another. To test this idea, computational and genomic methods will be employed. DNA arrays will be used to determine differential gene expression profiles, and cellular energy charge and DNA supercoiling levels will be monitored during aerobic to anaerobic growth transitions in the presence and absence of IHF. Computational predictions of IHF binding sites and predictions of the topological state of the E. coli chromosome will be used to analyze these data. It is expected that these experiments will identify additional operons that respond to energy charge and DNA supercoiling-mediated signals for further characterization. These experiments will further provide a wealth of information for future studies concerning the operon specific and global regulation of carbon and energy metabolism genes during aerobic to anaerobic growth transitions.

描述：（改编自研究者的摘要）：我们当前的 对大肠杆菌基因调控的理解提出了三个建议 控制层次：1）基础水平基因的全局控制 通过染色体结构表达； 2) 全局调节蛋白介导 刺激和调节的控制；以及，3) 操纵子特异性控制。第一个 DNA 超螺旋依赖性机制说明了控制水平 描述了 ILV 操纵子基础水平表达的协调 调节子。事实证明，这些控制可能会受到以下因素的影响： 染色体的拓扑结构，以及DNA结构蛋白 例如 IHF 能够调节这些的形成和位置 结构。本提案中描述的实验旨在进一步 描述了全球基因调控的第一级特征。建议 染色体结构对基因表达的调控受 细胞的能量电荷，反过来又受到营养和能量的影响 需要从一种生长状态转变为的环境条件 其他。为了测试这个想法，将采用计算和基因组方法。 DNA 阵列将用于确定差异基因表达谱，以及 细胞能量电荷和 DNA 超螺旋水平将在 在存在和不存在 IHF 的情况下，有氧到厌氧生长的转变。 IHF 结合位点的计算预测和 大肠杆菌染色体的拓扑状态将用于分析这些数据。 预计这些实验将鉴定出额外的操纵子 对能量电荷和 DNA 超螺旋介导的信号做出进一步反应 表征。这些实验将进一步提供丰富的 有关操纵子特异性和全局的未来研究信息 有氧到无氧过程中碳和能量代谢基因的调节 增长转变。

项目成果

Transcriptional coupling between the divergent promoters of a prototypic LysR-type regulatory system, the ilvYC operon of Escherichia coli.

原型 LysR 型调控系统（大肠杆菌的 ilvYC 操纵子）的不同启动子之间的转录耦合。

• DOI: 10.1073/pnas.96.25.14294
• 发表时间: 1999-12-07
• 期刊: Proceedings of the National Academy of Sciences of the United States of America
• 影响因子: 11.1
• 作者: K. Rhee;M. Opel;E. Ito;S. Hung;S. Arfin;G. W. Hatfield
• 通讯作者: G. W. Hatfield