Acquisition of a Varian 600 MHz High-Resolution NMR

获取 Varian 600 MHz 高分辨率 NMR

• 批准号: 6803723
• 负责人: Phil Crews
• 金额: $ 27.16万
• 依托单位: UNIVERSITY OF CALIFORNIA SANTA CRUZ
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-08-01 至 2008-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6803723
• 关键词: biomedical equipment purchase; chemical structure; chemical structure function; nuclear magnetic resonance spectroscopy

DESCRIPTION (provided by applicant): The aim of this grant application is to purchase a state-of-the-art 600 MHz NMR system equipped with a shielded magnet. It will greatly expand and modernize the campus Nuclear Magnetic Resonance (NMR) Laboratory housed in the Department of Chemistry and Biochemistry. Currently, the department has two NMR spectrometers - a 500 MHz instrument (NSF/NIH funded, 1995) and a 250 MHz instrument (FIMA funded, 1990). Obtaining high quality NMR data is a daily exercise in the research programs of the seven NIH grantees involved in this application. Their programs are investigating research topics that are of tremendous biomedical significance. The current and future initiatives will play a pivotal role in new discoveries that have many broader implications. We believe that the equipment proposed herein will also be an important tool for enhancing our teaching and research. There will be five major and four minor users of the proposed equipment. Two major users (Millhauser and Rohl) are probing the role of protein signaling and folding, which depend on proposed instrument. Three other major users (Crews, Konopelski, and Lokey) depend on the rapid characterization of complex bioorganic compound mixtures. The research of four other minor users involves: radical reagents (Braslau), new chiral synthesis tools (Singaram), metal ions in metalloenzymes and therapeutics (Mascharak), dynamics of active site iron in lipoxygenases (Holman). We propose to obtain a 600 MHz narrow bore NMR spectrometer and there are three companies manufacturing NMR systems operating at 14.1 T including the Bruker ADVANCE-ICE with an Ultrashield 600 Magnet, the JEOL ECA-600, and the Varian Unity Inova 600.These include (a) Magnet, (b) Console, (c) Pulse filed gradients, (d) Wave Form Generators, (e) Probes (with four probes as follows: (i) 1H{13C/X} PFG Tunable Triple Resonance with capabilities for single irradiation of 13C and X frequencies tunable from 15N to 31P; (ii) 1H{15N-31P} PFG indirect detect, the probe of choice for indirect detection of small and large biomolecules; (iii) 1H-19F/15N-31P 3mm PFG Dual Broadband, a probe useful for single resonance studies of sample limited compounds. (iv) A cryo cooled triple resonance probe for maximum sensitivity will be the 1H{13C/15N} 5mm PFG Triple), and (f) a Protasis Corporation CapNMR micro flow probe. The addition of a state-of-the-art 600 MHz instrument will greatly expand the range of experiments including essential capabilities such as those outlined in the research descriptions presented in the proposal. The instrument and its complement of probes will be essential for maintaining modern research with competitive throughput and the training of our students in a modern research environment.

描述（由申请人提供）：本赠款申请的目的是购买配备有屏蔽磁铁的最先进的600 MHz NMR系统。它将大大扩展和现代化化学和生物化学系所在的校园核磁共振（NMR）实验室。目前，该部门有两个NMR光谱仪 - 500 MHz仪器（NSF/NIH资助，1995年）和250 MHz仪器（FIMA资助，1990年）。获得高质量的NMR数据是该应用程序涉及的七个NIH授予者的研究计划的日常练习。他们的计划正在研究具有巨大生物医学意义的研究主题。当前和未来的举措将在具有更广泛含义的新发现中发挥关键作用。我们认为，本文提出的设备也将是增强我们的教学和研究的重要工具。 拟议的设备将有五个主要和4个次要用户。两个主要用户（Millhauser和Rohl）正在探测蛋白质信号传导和折叠的作用，这取决于建议的仪器。其他三个主要用户（Crews，Konopelski和Lokey）取决于复杂的生物有机化合物混合物的快速表征。对其他四个次要用户的研究涉及：激进试剂（Braslau），新手性合成工具（SINGARAM），金属酶和治疗剂中的金属离子（Mascharak），Lipoxygoxygenasase in Lipoxygoxyganase in Lipoxyganase in Active Site Irch的动力学（Holman）。 We propose to obtain a 600 MHz narrow bore NMR spectrometer and there are three companies manufacturing NMR systems operating at 14.1 T including the Bruker ADVANCE-ICE with an Ultrashield 600 Magnet, the JEOL ECA-600, and the Varian Unity Inova 600.These include (a) Magnet, (b) Console, (c) Pulse filed gradients, (d) Wave Form Generators, (e) Probes (with four探针如下：（i）1H {13C/x} PFG可调三重共振，具有13C的单个辐照，x频率从15n到31p;（ii）1H {15n-31p} 3mm PFG双重宽带，一种用于样品有限化合物的单共振研究的探针。添加最先进的600 MHz仪器将大大扩展实验范围，包括基本功能，例如该提案中提出的研究描述中概述的功能。该仪器及其补充探针对于通过竞争性吞吐量以及在现代研究环境中对学生的培训保持现代研究至关重要。