High-Throughput HomeLab System - R-AXIS++ Option

高通量家庭实验室系统 - R 轴选项

• 批准号: 6580715
• 负责人: JUNG-JA P KIM
• 金额: $ 33.5万
• 依托单位: MEDICAL COLLEGE OF WISCONSIN
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-05-01 至 2004-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6580715
• 关键词: X ray crystallography; biomedical equipment purchase; enzyme activity; flavoproteins; high throughput technology; nitric oxide synthase; oxidoreductase; peptide chemical synthesis; protein structure function

DESCRIPTION (provided by applicant): Protein crystallography continues to play an important role in the advances of biochemical research. This application proposes that our current unreliable instrumentation be updated to 21st century standards. This is not only important for the Medical College of Wisconsin, but for the entire southeastern Wisconsin region, since this is the only facility in this region for performing X-ray crystallographic experiments on biological macromolecules. The majority of the direct participants in this shared instrumentation come from the department of Biochemistry, although there is some participation from the department of Microbiology and the University of Wisconsin at Milwaukee. On going projects that will benefit from continued X-ray diffraction here at the Medical College include: (1) Structure and Function of a Flavoprotein Dehydrogenase, (2) Structure and Mechanism of a FMN and FAD-containing Enzymes, (3) Mevalonate metabolizing enzymes, (4) Structural Analysis of the Mannose 6- Phosphate Receptors, (5) Sorting of the IGF-II Receptor in Polarized Cells, (6) ATP-, Ubiquitin-dependent proteolysis, (7) Function of an interferon-induced ubiquitin homolog, (8) 4-Hydroxyphenylpyruvate Dioxygenase and Tyosinemia-1, (9) Design & Synthesis of Inhibitors of Human NOS Isoforms, (10) Design & Synthesis of Inhibitors of a-Glutamylcysteine Synthetase, (11) Roles for the divergent structural species of human lymphotactin, (12) Structure and Function of ExoS and related proteins, (13) Unique amino domains and AMP deaminase isoform diversity, (14) Proteases and Corneal Ulceration, (15) Maspin in the Cornea, (16) Structure and Function of Methylglyoxal Synthase, (17) Specific inhibitors for a non-specific enzyme: Aldose Reductase, and (18) Structure and Function of Phosphoribulokinase.

描述（由申请人提供）： 蛋白质晶体学在生化研究的进步中继续发挥着重要作用。该申请建议将我们当前不可靠的仪器更新到 21 世纪的标准。这不仅对威斯康星医学院很重要，而且对整个威斯康星东南部地区也很重要，因为这是该地区唯一对生物大分子进行 X 射线晶体学实验的设施。尽管微生物学系和威斯康星大学密尔沃基分校也有一些参与，但该共享仪器的大多数直接参与者来自生物化学系。医学院正在进行的项目将受益于持续的 X 射线衍射，包括：(1) 黄素蛋白脱氢酶的结构和功能，(2) 含 FMN 和 FAD 的酶的结构和机制，(3) 甲羟戊酸代谢酶，(4) 甘露糖 6-磷酸受体的结构分析，(5) 极化细胞中 IGF-II 受体的分选，(6) ATP 泛素依赖性蛋白水解，(7) 干扰素诱导的泛素同系物的功能，(8) 4-羟苯基丙酮酸双加氧酶和酪氨酸血症-1，(9) 人类 NOS 亚型抑制剂的设计与合成，(10) 设计与合成α-谷氨酰半胱氨酸合成酶抑制剂的合成，(11) 不同的作用人淋巴趋化素的结构种类，(12) ExoS 和相关蛋白质的结构和功能，(13) 独特的氨基结构域和 AMP 脱氨酶亚型多样性，(14) 蛋白酶和角膜溃疡，(15) 角膜中的 Maspin，(16) 结构甲基乙二醛合酶的结构和功能，（17）非特异性酶的特异性抑制剂：醛糖还原酶，以及（18）磷酸核糖激酶。