Core--Genomics

核心--基因组学

• 批准号: 7062892
• 负责人: JACK W FELL
• 金额: $ 3.46万
• 依托单位: UNIVERSITY OF MIAMI ROSENTEIL SCHOOL
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7062892
• 关键词: Dinoflagellates; algae; biomedical facility; functional /structural genomics; high throughput technology; marine biology; marine toxins; plant population genetics; protozoal genetics

The Rosenstiel School of Marine and Atmospheric Science has high capacity sequencing using an ABI 3730 (currently 48 capillaries will be upgraded to 96 capillaries) and genotyping using xMAP technology by Luminex, system. These systems are linked to robotics for high-throughput genomic analyses. Additionally, the facilities offer high capacity Q-PCR, gel electrophoresis, and thermal cycling. Support of the Genomics Core Facilities will allow us to train, analyze, and provide technical support to the Center for Ocean and Human Health researchers at a minimal cost (cost of reagents). In so doing, the investigators can apply modern high-throughput genomic techniques to better understand the biology, physiology and evolution of toxin producing algae and the distribution and occurrence of anthropogenic bacteria and viruses in marine habitats.

Rosenstiel海洋与大气科学学院使用ABI 3730（目前有48个毛细血管升级到96个毛细管）和使用XMAP Technology的Luminex，System，System，使用XMAP技术进行了高容量测序。这些系统与用于高通量基因组分析的机器人技术有关。此外，设施提供了高容量的Q-PCR，凝胶电泳和热循环。基因组学核心设施的支持将使我们能够以最低的成本（试剂成本）培训，分析和为海洋和人类健康研究人员提供技术支持。这样做，调查人员可以应用现代的高通量 基因组技术，以更好地了解产生毒素的生物学，生理和进化 藻类以及人类栖息地中人为细菌和病毒的分布和发生。