Core--Toxic Algal Culture

核心--毒藻培养

• 批准号: 7062893
• 负责人: KATHLEEN S REIN
• 金额: $ 3.19万
• 依托单位: UNIVERSITY OF MIAMI ROSENTEIL SCHOOL
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7062893
• 关键词: Dinoflagellates; algae; aquaculture; biomedical facility; flow cytometry; gene expression; marine toxins; microorganism mass culture; sample collection; tissue /cell culture

The proposed Toxic Algal Culture Core Facility will be located at Florida International University (FLU). This Facilities Core will provide field and laboratory support to the Toxic HAB research project (Brand/Rein), and the HAB Functional Genomics research project (Crawford/Hitchcock) as well as future Pilot Projects and other Oceans and Human Health investigators. Support will be in the form of the following; (1) Sample collection support and shipboard analysis. Continuous-flow bow pump water collection systems will be used to collect samples for flow-through chlorophyll fluorometry and flow cytometry. (2) Isolation of total population DNA from field collected samples for the preparation of 16S and 23S libraries to assess population diversity. (3) Isolation of RNA from field collected samples for gene expression studies. (4) Establishment of uni-algal and axenic (whenever possible) cultures of phytoplankton (dinoflagellates, prymnesiophytes and raphidophyts) by both traditional manual and automated cell-sorting flow cytometry methods. (5) Maintenance of clonal cultures and large scale culturing. (6) Collection of biomass from large scale cultures. (7) Growth of microorganisms under a variety of conditions for gene expression studies.

拟议的有毒藻类培养核心设施将位于佛罗里达国际大学（FLU）。该设施核心将为有毒 HAB 研究项目（Brand/Rein）、HAB 功能基因组学研究项目（Crawford/Hitchcock）以及未来的试点项目和其他海洋和人类健康研究人员提供现场和实验室支持。支持将采取以下形式； (1)样品采集支持和船上分析。连续流弓泵集水系统将用于收集样品，用于流通式叶绿素荧光测定法和流式细胞术。 (2)从现场采集的样本中分离总种群DNA，用于制备16S和23S文库以评估种群多样性。 (3)从现场采集的样品中分离RNA用于基因表达研究。 (4) 通过传统的手动和自动细胞分选流式细胞术方法建立浮游植物（甲藻、藻类和针藻类）的单一藻类和无菌（只要可能）培养物。 (5)克隆培养和大规模培养的维持。 (6)从大规模培养物中收集生物量。 (7) 微生物在多种条件下的生长，用于基因表达研究。