Core--Toxic Algal Culture

核心--毒藻培养

• 批准号: 6751039
• 负责人: KATHLEEN S REIN
• 金额: $ 3.41万
• 依托单位: UNIVERSITY OF MIAMI ROSENTEIL SCHOOL
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-09-01 至 2008-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6751039
• 关键词: Dinoflagellates; algae; aquaculture; biomedical facility; flow cytometry; gene expression; marine toxins; microorganism mass culture; sample collection; tissue /cell culture

The proposed Toxic Algal Culture Core Facility will be located at Florida International University (FLU). This Facilities Core will provide field and laboratory support to the Toxic HAB research project (Brand/Rein), and the HAB Functional Genomics research project (Crawford/Hitchcock) as well as future Pilot Projects and other Oceans and Human Health investigators. Support will be in the form of the following; (1) Sample collection support and shipboard analysis. Continuous-flow bow pump water collection systems will be used to collect samples for flow-through chlorophyll fluorometry and flow cytometry. (2) Isolation of total population DNA from field collected samples for the preparation of 16S and 23S libraries to assess population diversity. (3) Isolation of RNA from field collected samples for gene expression studies. (4) Establishment of uni-algal and axenic (whenever possible) cultures of phytoplankton (dinoflagellates, prymnesiophytes and raphidophyts) by both traditional manual and automated cell-sorting flow cytometry methods. (5) Maintenance of clonal cultures and large scale culturing. (6) Collection of biomass from large scale cultures. (7) Growth of microorganisms under a variety of conditions for gene expression studies.

拟议的有毒藻类培养核心设施将位于佛罗里达国际大学（FLU）。该设施核心将为有毒HAB研究项目（Brand/Rein）以及HAB功能基因组学研究项目（Crawford/Hitchcock）以及未来的飞行员项目以及其他海洋和人类健康调查人员提供现场和实验室支持。支持将以以下形式进行； （1）样本收集支持和船上分析。连续流弓泵水收集系统将用于收集流通叶绿素荧光法和流式细胞术的样品。 （2）将总人口DNA与收集的样品分离，以制备16S和23S库，以评估人口多样性。 （3）从收集的样品中分离RNA进行基因表达研究。 （4）通过传统的手动和自动化细胞分类流式细胞术方法，建立浮游植物（鞭毛藻，prymnesiophytes和raphidophyts）的单藻和轴承培养物。 （5）维持克隆文化和大规模培养。 （6）从大规模培养物中收集生物量。 （7）在各种条件下，微生物的生长用于基因表达研究。