Core--Genomics

核心--基因组学

• 批准号: 6751038
• 负责人: JACK W FELL
• 金额: $ 3.49万
• 依托单位: UNIVERSITY OF MIAMI ROSENTEIL SCHOOL
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-09-01 至 2008-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6751038
• 关键词: Dinoflagellates; algae; biomedical facility; functional /structural genomics; high throughput technology; marine biology; marine toxins; plant population genetics; protozoal genetics

The Rosenstiel School of Marine and Atmospheric Science has high capacity sequencing using an ABI 3730 (currently 48 capillaries will be upgraded to 96 capillaries) and genotyping using xMAP technology by Luminex, system. These systems are linked to robotics for high-throughput genomic analyses. Additionally, the facilities offer high capacity Q-PCR, gel electrophoresis, and thermal cycling. Support of the Genomics Core Facilities will allow us to train, analyze, and provide technical support to the Center for Ocean and Human Health researchers at a minimal cost (cost of reagents). In so doing, the investigators can apply modern high-throughput genomic techniques to better understand the biology, physiology and evolution of toxin producing algae and the distribution and occurrence of anthropogenic bacteria and viruses in marine habitats.

罗森斯蒂尔海洋与大气科学学院拥有使用 ABI 3730 的高容量测序（目前 48 个毛细管将升级到 96 个毛细管），并使用 Luminex 系统的 xMAP 技术进行基因分型。这些系统与机器人相连，用于高通量基因组分析。此外，这些设施还提供高容量 Q-PCR、凝胶电泳和热循环。基因组学核心设施的支持将使我们能够以最低的成本（试剂成本）为海洋与人类健康中心的研究人员提供培训、分析和技术支持。这样做，研究人员可以应用现代高通量 基因组技术可以更好地了解毒素产生的生物学、生理学和进化 藻类以及海洋生境中人为细菌和病毒的分布和发生。