Juxtacellular characterization of V1 neurons

V1 神经元的细胞旁特征

基本信息

批准号：
6877023
负责人：
MICHAEL J HAWKEN
金额：
$ 11.48万
依托单位：
NEW YORK UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2004
资助国家：
美国
起止时间：
2004-04-01 至 2007-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6877023
关键词：
Cercopithecidae Macaca biocytin brain electrical activity brain mapping cell morphology cell population study electronic recording system electrophysiology microelectrodes neural information processing neural transmission neuroanatomy single cell analysis technology /technique development visual cortex visual pathways voltage /patch clamp

项目摘要

(Revised Abstract) Description: The cerebral cortex is vitally important for perception and thought. Understanding how the cortex works is one of the great unsolved scientific problems. We have been studying the primary visual cortex, V1, in order to understand its role in vision and also because the V1 is one of the best-understood regions of the cerebral cortex. On the one hand we often have detailed knowledge of neuronal firing in V1 to an array of visual stimuli, obtained from extracellular recording. On the other hand, from anatomical studies, we know about the intricate neuronal architecture and the patterns of synaptic connections both within V1 and between V1 and extra-striate cortex. In order to understand such a complicated neuronal network, modeling how cortical neurons interact within the network is crucial. At present, there is one link that is missing that if present would enable much more realistic modeling of the cortex: the link between structure and function. In this application we are proposing experiments to pilot an extension of juxtacellular recording using a "loose" patch method (JC-LP, juxtacellular-loose patch). Development of this technique would allow us to characterize and stain neurons in visual cortex in vivo and would thereby provide the link between function and structure.

（修订摘要）描述：大脑皮层对于感知和思想至关重要。了解皮质的工作原理是尚未解决的科学问题之一。我们一直在研究主要的视觉皮层V1，以了解其在视觉中的作用，也是因为V1是大脑皮层最受欢迎的区域之一。一方面，我们经常对从细胞外记录获得的V1中的神经元发射到一系列视觉刺激中有详细的知识。另一方面，从解剖学研究中，我们了解了V1内以及V1和V1和外部构成皮质之间的复杂神经元结构以及突触连接的模式。为了理解这种复杂的神经元网络，建模皮层神经元如何在网络中相互作用至关重要。目前，有一个链接缺少，如果存在将使皮质的更现实的建模：结构和功能之间的链接。在此应用中，我们提出了实验，以使用“松散”贴片方法（JC-LP，叶循环 - 循环）进行并列记录的扩展。该技术的开发将使我们能够在体内表征和染色神经元，从而提供功能与结构之间的联系。