Research Services Core

研究服务核心

基本信息

批准号：
10495209
负责人：
Adriana Galvan
金额：
$ 29.12万
依托单位：
EMORY UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-09-29 至 2026-07-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10495209
关键词：
1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine Acute Anatomy Animal Model Animals Antiparkinson Agents Behavior Behavior assessment Behavioral Bioinformatics Biometry Brain Caring Cells Chronic Clinical Conceptions Consultations Data Data Analyses Denervation Dopamine Dose Electrodes Electrophysiology (science)Evaluation Experimental Designs Feedback Fiber Optics Genes Health Services Accessibility Histologic Histological Techniques Histology Human Resources Image Analysis Immunohistochemistry Injections Levodopa Location MPTP model MPTP treatment Macaca mulatta Methods Microscopy Modeling Monkeys Mus Neurotoxins Opsin Oxidopamine Parkinson Disease Parkinsonian Disorders Pattern Penetration Procedures Process Proteins Public Health Schools Reproducibility Research Research Design Research Personnel Research Project Grants Research Project Summaries Resources Rhesus Risk Services Spinal Standardization Statistical Data Interpretation Techniques Testing Time Tracer Transgenes Universities Viral Vector Virus biocytin brain tissue data sharing design digital imaging experience experimental analysis experimental study instructor light microscopy nervous system disorder optogenetics parkinsonian animal parkinsonian non-human primate professor reconstruction sensor statistical service tissue processing transgene expression voltage

项目摘要

Project Summary – Research Services Core (Core B) The Research Services Core (Core B) will be an essential part of the Emory Udall Center. The Core will have three functions: (1) to prepare and process the brain tissue from experimental animals generated by the Center’s projects, (2) to treat Rhesus monkeys systemically with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) and evaluate the resulting parkinsonism in a standardized manner, and (3) to provide biostatistical services and consultation for all projects in the Center. The Core will provide standardized histology services to researchers using animal models of parkinsonism (projects 1-3). These services will include the use of immunohistochemical and microscopy approaches to evaluate the degree of dopamine depletion in 6-hydroxydopamine (6-OHDA) or MPTP-treated animals (projects 1-3); histological reconstructions of recording electrode trajectories (projects 1 and 2); analysis of the degree and pattern of expression of transgenes (opsins, fluorescent proteins or voltage sensors) after virus injections (projects 1-3). These services will be indispensable to interpret the functional experiments and will help to compare and share the results among the projects. Similarly, all MPTP-treated parkinsonian monkeys that will be used in projects 2 and 3 will be treated and evaluated using standardized procedures by Core B personnel. This will help to integrate and compare data generated from these projects. Finally, the Core will provide the services of a biostatistician that will advise and provide feedback on statistical aspects of the projects. All four projects in the Udall Center will have access to these services, from the conception and design of the experiments to the statistical analysis of experimental data. By providing these services, the Core will help to free up time and resources from the research projects. In addition, center research will benefit from the use of standardized, well-establish anatomical and behavioral assessment techniques, and the biostatistical support will promote rigor and reproducibility in all projects.

项目摘要 – 研究服务核心（核心 B）研究服务核心（核心 B）将成为埃默里尤德尔中心的重要组成部分。三大功能：（1）制备和加工中心产生的实验动物脑组织项目，(2) 用 1-甲基-4-苯基-1,2,3,6-四氢吡啶 (MPTP) 系统治疗恒河猴并以标准化方式评估由此产生的帕金森症，以及（3）提供生物统计服务和该中心将为研究人员提供标准化的组织学服务。使用帕金森病动物模型（项目 1-3）。这些服务将包括使用免疫组织化学。和显微镜方法来评估 6-羟基多巴胺 (6-OHDA) 或 MPTP 处理的动物（项目 1-3）；记录电极轨迹的组织学重建（项目 1） 2) 分析转基因的表达程度和模式（视蛋白、荧光蛋白或电压）病毒注入后（项目 1-3），这些服务对于解释功能是必不可少的。实验，并将有助于在所有经过 MPTP 处理的项目之间进行类似的比较和共享结果。将使用标准化方法对项目 2 和 3 中使用的帕金森猴进行治疗和评估核心 B 人员制定的程序，这将有助于整合和比较这些项目生成的数据。最后，核心将提供生物统计学家的服务，为统计提供建议和反馈尤德尔中心的所有四个项目都可以从以下机构获得这些服务。通过提供这些，从实验的构思和设计到实验数据的统计分析。服务，核心将有助于从研究项目中释放时间和资源。将受益于标准化、完善的解剖学和行为评估技术的使用，并且生物统计支持将促进所有项目的严谨性和可重复性。