Ultrasonic Imaging of LIOB in Dendrimer Nanocomposites

树枝状聚合物纳米复合材料中 LIOB 的超声成像

• 批准号: 6867835
• 负责人: Matthew O'Donnell
• 金额: $ 22.95万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-01 至 2007-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6867835
• 关键词: bioimaging /biomedical imaging; chemical structure function; lasers; molecular /cellular imaging; nanotechnology; photobiology; polymers; ultrasonography

DESCRIPTION (provided by applicant): The central aim of this proposal is to understand ultrafast light-DNC interactions as monitored by high frequency ultrasound. In particular, we will use ultrasonic micrsocopy to monitor the photodisruption process transducing site-targeted nanoparticles into a detectable microbubble. Our short-term goal is to detect molecular agents targeted to squamous cell cancers and to monitor therapy applied to these cells. We propose to investigate two photodisruption regimes: one near threshold in which the UOB process can be carefully controlled to produce detectable microbubbtes with little cellular injury (i.e., minimally invasive); the second at a different set of optical parameters where the UOB processes can be highly destructive, killing labeled cells for therapeutic purposes, ff both regimes can be established, then ultrasonic detection of DNC promoted photodisruption can provide a sensitive tool for both site-targeted molecular imaging and molecular therapeutics. It is the aim, therefore, of the work proposed here to address the following issues in detail. 1.) Characterize LIOB and resultant microbubbles in water, water-based gels, and tissue culture using high-frequency ultrasound. In particular, the ultrasound system will monitor photodisruption thresholds, system parameters for minimally invasive transient bubble creation, system parameters for invasive and stable bubble creation, system parameters determining bubble size, and system parameters determining bubble temperature. 2.) Determine the necessary composition and structure of DNC particles that have minimal LIOB thresholds. Detailed structural studies will be performed on all compositions showing enhanced breakdown characteristics. 3.) Determine the range of optical parameters controlling LIOB thresholds and photodisruption characteristics in DNC solutions and DNC loaded tissue-equivalent gelatin phantoms, including wavelength, optical fluence per pulse, repetition rate, and total number of pulses. If these studies demonstrate that we can control DNC-promoted LIOB to operate either as a minimally invasive sensor or a highly localized disruptor, and we can sensitively monitor both processes with high frequency ultrasound, we will develop an RO1 proposal for site-targeted molecular imaging and therapy monitoring of squamous cell cancers, a rapidly growing and very important clinical problem.

描述（由申请人提供）： 该提案的核心目的是了解高频超声监测的超快光DNC相互作用。特别是，我们将使用超声波微量学检查来监测将位点靶向纳米颗粒转换为可检测的微泡的光点过程。我们的短期目标是检测靶向鳞状细胞癌的分子剂并监测应用于这些细胞的治疗。我们建议研究两个光散发状态：一个接近阈值，可以仔细控制UOB过程，以产生几乎没有细胞损伤的可检测的微生物（即微创）；第二个在不同的光学参数上，UOB过程可能具有高度破坏性，可以为治疗目的而杀死标记的细胞，FF可以建立这两种状态，然后可以建立FF，然后对DNC的超声检测促进的光dipodisction促进促进的光diposphitions可以为两个敏感的工具提供敏感的工具，以构成网站靶向的分子成像和分子治疗方法。 因此，这里提出的工作旨在详细解决以下问题的目的。 1.）使用高频超声来表征水，水基凝胶和组织培养中的微泡。特别是，超声系统将监视光界中断阈值，用于微创瞬态气泡创建的系统参数，侵入性和稳定气泡创建的系统参数，确定气泡大小的系统参数以及确定气泡温度的系统参数。 2.）确定具有最小LIOB阈值的DNC颗粒的必要组成和结构。详细的结构研究将对显示出增强分解特征的所有组成进行。 3.）确定控制LIOB阈值和DNC溶液中的光段特性的光学参数范围和DNC加载的组织等效的明胶幻象（包括波长，每个脉冲的光通量，重复率和脉冲总数）。 如果这些研究表明我们可以控制DNC启动的LIOB作为一种微创传感器或高度局部的破坏者操作，并且我们可以通过高频超声检查敏感地监视这两个过程，我们将开发一个针对现场的靶标分子成像和监测快速生长型型型诸如快速临床问题的RO1提案，并具有重要的临床问题。