Characterizing Tissue Development Under Biaxial Loading

双轴载荷下组织发育的表征

• 批准号: 6900912
• 负责人: ALVIN T YEH
• 金额: $ 20.37万
• 依托单位: TEXAS ENGINEERING EXPERIMENT STATION
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-01 至 2007-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6900912
• 关键词: bioengineering /biomedical engineering; bioimaging /biomedical imaging; biomechanics; biomedical equipment development; controlled environment chamber; crosslink; extracellular matrix; fluorescence microscopy; intravital microscopy; method development; optics; tissue /cell culture; tissue engineering

DESCRIPTION (provided by applicant): We propose to design and construct a custom culture chamber system which can provide controlled mechanical tissue culture environments and be mounted on a microscope stage used for nonlinear optical microscopy (NLOM). A multidisciplinary team has been assembled, integrating expertise in mechanobiology, nonlinear optics and tissue engineering. The culture chamber system will be the first of its kind, designed for longitudinal studies of tissue response to controlled mechanical loads using NLOM to evaluate microstructure organization and matrix content and stress-strain measurements to characterize bulk mechanical properties on individual tissue specimens. By combining biomechanical measurements with constituent specific, non-destructive, intravital imaging, fundamental structure-function relationships may be directly studied and delineating biological events responsible for translating cellular expression profiles into bulk tissue response becomes feasible. In the two year project period, we propose to build this novel culture chamber system and establish protocols for acquiring quantitative measures of extracellular matrix parameters using NLOM. These experimental protocols will be designed to monitor evolving changes in tissue microstructure and bulk mechanical properties over a three week period. These quantitative NLOM measurements will be correlated with bulk biomechanical properties on the exact same tissue specimen and will provide experimental parameters for our developing mathematical theory of tissue growth and remodeling. This novel experimental platform will form the basis for novel and innovative studies in mechanobiology.

描述（由申请人提供）：我们建议设计和构建一个自定义培养室系统，该系统可以提供受控的机械组织培养环境，并安装在用于非线性光学显微镜（NLOM）的显微镜阶段。一支多学科团队已经组装，整合了机械生物学，非线性光学和组织工程方面的专业知识。培养室系统将是同类产品中的第一个，旨在使用NLOM对组织对受控机械负荷的纵向研究进行纵向研究，以评估微观结构组织和基质含量和应力 - 应变测量，以表征单个组织标本上的大量机械性能。通过将生物力学测量与组成的特异性，非破坏性，内内室成像相结合，可以直接研究基本的结构 - 功能关系，并描述负责将细胞表达谱转化为块状组织响应的生物事件变得可行。在为期两年的项目期间，我们建议建立这个新型的培养室系统，并建立使用NLOM来获取细胞外基质参数定量测量的方案。这些实验方案将旨在监测三周内组织微观结构和大量机械性能的变化。这些定量的NLOM测量结果将与完全相同的组织样品上的块状生物力学特性相关，并将为我们发展的组织生长和重塑的数学理论提供实验参数。这个新颖的实验平台将构成机械生物学新颖和创新研究的基础。