Noninvasive imaging of tissue-engineered blood vessels

组织工程血管的无创成像

• 批准号: 7035994
• 负责人: MARK Andreas HAIDEKKER
• 金额: $ 19.69万
• 依托单位: UNIVERSITY OF MISSOURI-COLUMBIA
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-06-15 至 2007-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7035994
• 关键词: angiography; bioengineering /biomedical engineering; bioimaging /biomedical imaging; biomechanics; biomedical equipment development; bioreactors; blood vessel transplantation; image processing; optical tomography; tissue engineering

DESCRIPTION (provided by applicant): In this project we will develop novel imaging technology for quality control of tissue-engineered blood vessels (TEBV). Recent developments in the field of tissue engineering led to the development of completely biological living vascular graft that solves major problems associated with synthetic materials such as inflammation and infection. Since this type of vascular graft is not produced in large batches, but rather on a patient-to-patient basis, close monitoring of the development process is paramount. Unlike synthetic grafts, mechanical testing of duplicate grafts is very costly and not very reliable. Hence, there is growing demand for noninvasive methods to predict biomechanical properties. However, due to the novelty of the manufacturing process, no suitable imaging modalities exist. The purpose of this project is to develop and refine methods based on transillumination optical tomography (OT) and optical coherence tomography (OCT) for thin tissue samples (vascular grafts are 300 - 500 Lim thick), and relate the images to biomechanical properties. In this project, the OT and OCT scanners will be developed. This includes the acquisition control and the development of image reconstruction and enhancement algorithms. The goal is to achieve optimum signal-to-noise ratio at a resolution of 10 pm and short (5-20 minute) scanning times. Specialized bioreactors will be developed that allow to obtain images of the growing vessels inside the bioreactor without breaking sterility, allowing for routine examination. We will determine the capabilities of the new modalities in comparison with histology and with respect to biomechanical testing. The proposed OT and OCT imaging techniques are not limited to TEBV. Additional applications include conventional grafts as well as other tissue engineering products, such as artificial skin and cartilage. The results of this project will provide novel imaging methods that will accelerate the development and clinical availability of tissue engineering products.

描述（由申请人提供）：在此项目中，我们将开发新颖的成像技术，以控制组织工程血管（TEBV）的质量控制。组织工程领域的最新发展导致了完全生物活血管移植的发展，从而解决了与炎症和感染等合成材料相关的主要问题。由于这种类型的血管移植物不是在大批处理中产生的，而是在患者到患者的基础上产生，因此对开发过程的密切监测至关重要。与合成移植物不同，重复移植的机械测试非常昂贵，而且不是很可靠。因此，对预测生物力学特性的非侵入性方法的需求不断增长。但是，由于制造过程的新颖性，不存在合适的成像方式。该项目的目的是基于透明光学层析成像（OT）（OT）和光学相干断层扫描（OCT）的薄组织样品（血管移植物为300-500 lim厚），并将图像与生物力学特性相关联。在该项目中，将开发OT和OCT扫描仪。这包括获取控制以及图像重建和增强算法的开发。目的是在10 pm和短（5-20​​分钟）扫描时间的分辨率下达到最佳的信噪比。将开发专门的生物反应器，以获取生物反应器内生长血管的图像，而不会破坏无菌性，从而可以进行常规检查。与组织学相比，我们将确定新方式的能力以及生物力学测试。提出的OT和OCT成像技术不限于TEBV。其他应用包括常规移植物以及其他组织工程产品，例如人造皮肤和软骨。该项目的结果将提供新颖的成像方法，以加速组织工程产品的开发和临床可用性。