DEVELOPMENT OF A FLUORESCENCE-BASED BIOFLUID VISCOMETER

基于荧光的生物流体粘度计的开发

• 批准号: 6909444
• 负责人: MARK Andreas HAIDEKKER
• 金额: $ 21.33万
• 依托单位: UNIVERSITY OF MISSOURI-COLUMBIA
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-01 至 2006-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6909444
• 关键词: biomedical equipment development; body fluid viscosity; fluorescent dye /probe; interstitial; measurement; plasma

DESCRIPTION (provided by applicant): To date, fluid viscosity is obtained by mechanical means. Fluids are sheared, and the resistance against shear is related to viscosity. All mechanical methods have in common that the measurement process is time-consuming and requires relatively large amounts of fluid. The measurement apparatus needs scrupulous cleaning, which increases time requirements for measurement series. When measuring biofluids, which contain large amounts of proteins and/or colloids, protein deposition at the instrument surface introduces errors, as does the protein interaction at the liquid/air interface. Fluorescent molecular rotors, molecules that change their fluorescence quantum yield with the viscosity of the environment, allow viscosity measurements in biofluids without the above disadvantages. A viscometer is proposed in this development grant that allows viscosity measurements of biofluids based on fluorescent molecular rotors. The first phase of this grant addresses specific issues such as rotor-protein interaction, measurability of various colloids, fluid turbidity and absorption. It will be attempted to find a specific molecule and a specific measurement technique that allows viscosity measurements with a precision that exceeds that of standard viscometers. During the second phase, two prototypes of fluorescence-based viscometers will be developed. One aims at high accuracy for laboratory usage, and the second will be a fast, low-volume battery-powered field-usable device. Both devices will have applications in research areas that involve blood plasma, lymphatic fluid and interstitial fluid viscosity. One additional application is the monitoring of blood replacement using high-viscosity plasma expanders in trauma medicine.

描述（申请人提供）：迄今为止，流体粘度是通过 机械手段。流体是剪切的，对剪切的阻力为 与粘度有关。所有机械方法的共同点 测量过程是耗时的，需要相对较大的 体液。测量设备需要仔细清洁，这会增加 测量系列的时间要求。测量生物流体时 包含大量蛋白质和/或胶体，蛋白质沉积在 仪表表面引入了错误，蛋白质相互作用也在 液体/空气界面。荧光分子转子，变化的分子 它们的荧光量子产率与环境的粘度，允许 没有上述缺点的生物流体中的粘度测量。一个 在此开发赠款中提出了粘度计，该赠款允许粘度 基于荧光分子转子的生物流体的测量。第一个 该赠款的阶段解决了特定问题，例如转子蛋白 相互作用，各种胶体的可测量性，流体浊度和 吸收。它将尝试找到特定的分子和一个特定的分子 测量技术允许粘度测量精确地测量 超过标准粘度器的。在第二阶段，两个原型 将开发基于荧光的粘度；一个目标是 实验室使用的准确性，第二个将是快速，小体积的 电池驱动的现场使用设备。两种设备都将在 涉及血浆，淋巴液和间质的研究区域 流体粘度。另一种应用是血液的监测 使用高粘度等离子体扩张器中的创伤医学中的替换。