Interferon regulation of T-bet in airway smooth muscle

干扰素对气道平滑肌 T-bet 的调节

• 批准号: 6945069
• 负责人: Cherie Singer
• 金额: $ 11.75万
• 依托单位: UNIVERSITY OF NEVADA RENO
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-30 至 2009-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6945069
• 关键词: bronchus; cell cycle; cell line; cell migration; cytoskeletal proteins; dogs; helper T lymphocyte; human genetic material tag; interferon gamma; muscle contraction; muscle function; muscle proteins; protein biosynthesis; respiratory muscles; respiratory system; smooth muscle; trachea; transcription factor

DESCRIPTION (provided by applicant): A Mentored Career Development Award will allow me to become an innovative, recognized investigator and instructor by expanding my research skills, enhancing my ideas for my own research program in airway smooth muscle biology, and providing me with opportunities to continue to develop expertise in teaching through the outlined career development and research plans. I believe that my previous training and research experience at both large and small universities has given me an excellent foundation in which to begin an independent research career. As a graduate student, I endured a rigorous program of study that emphasized critical and analytical thinking, oral communication and presentation skills, and research excellence in the neurosciences. As a post-doctoral fellow, I developed an interest in the role of airway smooth muscle (ASM) in inflammatory events that contribute to airway diseases and took advantage of opportunities to gain teaching experience. I continue my interest in airway smooth muscle biology with this proposal by examining the T-box transcription factor T-bet in ASM. The proposed career development plan will facilitate my goals through intellectual interactions with colleagues, students and my mentor, Dr. William T. Gerthoffer, along with participation in structured activities including University service, attendance at scientific meetings, and involvement in teaching, career development and laboratory management workshops and seminars. The research project described here examines the hypothesis that ASM expression of T-bet participates in the development of a secretory smooth muscle cell phenotype that affects the inflammatory response by increasing T helper cell 1 (Th1) cytokine secretion. The experiments described will examine IFNgamma regulation of T-bet expression in ASM, determine whether T-bet expression contributes to the development of a secretory smooth muscle cell phenotype by upregulating Th1 cytokines, and addresses whether T-bet alters the functions of ASM cells such as proliferation, migration and contraction. There are few places that I would have the opportunity to interact with faculty that are as recognized for their expertise in smooth muscle cell biology than at the University of Nevada School of Medicine. This award will allow me to begin to ask novel questions about the secretory phenotype of ASM in this supportive research environment that is committed to furthering junior faculty development.

描述（由申请人提供）： 指导职业发展奖将使我能够扩展我的研究技能，增强我对自己的气道平滑肌生物学研究项目的想法，并为我提供继续发展教学专业知识的机会，从而成为一名创新的、公认的研究者和讲师。概述的职业发展和研究计划。我相信我之前在大型和小型大学的培训和研究经验为我开始独立的研究生涯奠定了良好的基础。作为一名研究生，我接受了严格的学习计划，强调批判性和分析性思维、口头沟通和演讲技巧以及神经科学研究的卓越性。作为一名博士后研究员，我对气道平滑肌 (ASM) 在导致气道疾病的炎症事件中的作用产生了兴趣，并利用机会获得了教学经验。通过研究 ASM 中的 T-box 转录因子 T-bet，我继续对气道平滑肌生物学的兴趣。拟议的职业发展计划将通过与同事、学生和我的导师 William T. Gerthoffer 博士的智力互动，以及参与结构化活动（包括大学服务、参加科学会议以及参与教学、职业发展和培训）来促进我的目标。实验室管理讲习班和研讨会。这里描述的研究项目检验了这样的假设：T-bet 的 ASM 表达参与分泌性平滑肌细胞表型的发展，该表型通过增加 T 辅助细胞 1 (Th1) 细胞因子的分泌来影响炎症反应。所描述的实验将检查 ASM 中 T-bet 表达的 IFNgamma 调节，确定 T-bet 表达是否通过上调 Th1 细胞因子促进分泌性平滑肌细胞表型的发展，并解决 T-bet 是否改变 ASM 细胞的功能，例如如增殖、迁移和收缩。很少有地方比内华达大学医学院更有机会与那些在平滑肌细胞生物学方面的专业知识得到认可的教师互动。这个奖项将使我能够在这个致力于促进初级教师发展的支持性研究环境中开始提出有关 ASM 分泌表型的新问题。