COBRE: UNR: MOLECULAR BIOLOGY CORE (B)

COBRE：UNR：分子生物学核心 (B)

• 批准号: 7959485
• 负责人: Cherie Singer
• 金额: $ 24.04万
• 依托单位: UNIVERSITY OF NEVADA RENO
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-06-01 至 2010-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7959485
• 关键词: Adenoviruses; Centers of Research Excellence; Chloride Channels; Co-Immunoprecipitations; Computer Retrieval of Information on Scientific Projects Database; DNA; Disease; Funding; Grant; Institution; Molecular Biology; Nevada; Production; Protein Analysis; Proteins; Proteomics; Recombinant Proteins; Recombinants; Research; Research Personnel; Resources; Role; Sequence Determination; Source; Time; United States National Institutes of Health; Universities; Work; assay development; design; expression cloning; protein profiling

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Core B is the Molecular Biology Core. This core will serve all of the cobre projects by providing general cloning and expression of recombinant proteins, recombinant adenovirus production, Southern, Northern and Western analysis. In addition, Core B will design primers and probes for real-time PCR and DNA seqeuence analysis along with co-immunoprecipitation assay development. In addition, this core will perform all preparative work required by the COBRE projects for the subsequent analysis and identification of proteins and protein profiling. The University of Nevada has a well-established proteomics facility, the Nevada Proteomics Center, that can adequately perform all the necessary protein analysis such as protein mass and sequence determination.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 核心 B 是分子生物学核心。 该核心将通过提供重组蛋白的一般克隆和表达、重组腺病毒生产、Southern、Northern 和 Western 分析来服务于所有 cobre 项目。 此外，Core B 将设计用于实时 PCR 和 DNA 序列分析以及免疫共沉淀测定开发的引物和探针。 此外，该核心还将执行 COBRE 项目所需的所有准备工作，用于随后的蛋白质分析和鉴定以及蛋白质谱分析。 内华达大学拥有完善的蛋白质组学设施，即内华达蛋白质组学中心，可以充分执行所有必要的蛋白质分析，例如蛋白质质量和序列测定。