Developing Functional Tools for Digital Brain Atlases

开发数字脑图谱的功能工具

• 批准号: 6934427
• 负责人: Jeffrey Redwine
• 金额: $ 20万
• 依托单位: NEUROME, INC.
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-06-01 至 2006-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6934427
• 关键词: bioimaging /biomedical imaging; brain imaging /visualization /scanning; brain mapping; computer program /software; computer system design /evaluation; digital imaging; gene expression; genetically modified animals; in situ hybridization; laboratory mouse; microarray technology; three dimensional imaging /topography

DESCRIPTION (provided by applicant): The completion of the mouse genome and the resultant ability to create genetically altered mice have made the mouse the dominant animal model for brain research. However, the limited knowledge of mouse brain connectivity, neurochemistry and physiology, along with existing variations among multiple mouse strains has presented a clear need for more efficient and coordinated information on the mouse brain. Digital brain atlases can help fill this need by providing an invaluable and comprehensive neuroanatomical resource, while also offering access to a wealth of information that can increase the rate and accuracy of data analysis. Yet, the creation of digital atlases is hindered by time consuming and labor intensive aspects of this process. Neurome, Inc. has begun to address these issues in the course of developing a 3-dimensional, digital C57BL/6 mouse brain atlas annotated with all major nuclei, myelinated tracts, and ventricles. Based on this preliminary work, the overall goal of this proposal is to develop, enhance and utilize our technology in creating tools that will accelerate the production of digital brain atlases and expand their applications. In Phase I, the goals are to 1) develop and validate software to accelerate the graphical delineation of brain regions on digital images and 2) assess an initial application of the digital atlas as a display tool for gene expression data from either in situ hybridization or DMA microarray analyses. In Phase II, we will integrate our technology with that of the TeraGenomics analysis system, managed by Information Management Consultants, and thus establish a channel for commercialization. By expanding the capabilities of digital atlases to extract and display data from various different experimental sources, we will provide a powerful and sophisticated neuroinformatics tool for more rapid and reproducible data analysis, with enormous value and marketability for anyone working on the mouse brain.

描述（由申请人提供）：小鼠基因组的完成以及由此产生的基因改造小鼠的能力使小鼠成为大脑研究的主要动物模型。然而，由于对小鼠大脑连接、神经化学和生理学的了解有限，加上多种小鼠品系之间存在的差异，显然需要更有效、更协调的小鼠大脑信息。数字大脑图谱可以通过提供宝贵且全面的神经解剖学资源来帮助满足这一需求，同时还提供丰富的信息，从而提高数据分析的速度和准确性。然而，数字地图集的创建受到这一过程耗时和劳动密集型方面的阻碍。 Neurome, Inc. 已开始在开发 3 维数字 C57BL/6 小鼠大脑图谱的过程中解决这些问题，该图谱标注有所有主要核、有髓神经束和脑室。基于这项初步工作，该提案的总体目标是开发、增强和利用我们的技术来创建工具，以加速数字大脑图谱的生产并扩展其应用。在第一阶段，目标是 1) 开发和验证软件，以加速数字图像上大脑区域的图形描绘，2) 评估数字图谱作为原位杂交或基因表达数据显示工具的初始应用。 DMA 微阵列分析。在第二阶段，我们将把我们的技术与由信息管理顾问管理的TeraGenomics分析系统的技术相结合，从而建立商业化的渠道。通过扩展数字图谱从各种不同实验来源提取和显示数据的能力，我们将提供强大而复杂的神经信息学工具，以实现更快速和可重复的数据分析，为任何研究小鼠大脑的人带来巨大的价值和适销性。