Molecular basis of reproductive isolation in Drosophila

果蝇生殖隔离的分子基础

• 批准号: 6995009
• 负责人: AMANDA J MOEHRING
• 金额: $ 4.83万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-08-01 至 2008-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6995009
• 关键词: Drosophilidae; arthropod genetics; developmental genetics; evolution; fertility; gene expression; genetically modified animals; microarray technology; postdoctoral investigator; quantitative trait loci; sex behavior; spermatogenesis

DESCRIPTION (provided by applicant): The process by which evolutionary forces act upon standing genetic variation to cause species formation remains one of the greatest unanswered questions in biology. Two primary goals of research on the speciation process are determining the genetic changes underlying reproductive barriers and identifying the evolutionary forces driving their divergence. To test the multitude of hypotheses regarding speciation, it is critical to first identify the genes responsible for species divergence, knowledge that is currently extremely limited as only four genes affecting reproductive isolation have been identified. This proposal focuses on using gene expression assays and transgenics to identify the genetic basis of 1) behavioral isolation preventing successful copulation through characterization of candidate genes that were previously indicated through quantitative trait locus mapping affecting copulation occurrence between Drosophila simulans and D. mauritiana and 2) the formation of sterile offspring through identification of genes responsible for hybrid sterility between D. simulans and its sibling species D. mauritiana and D. sechellia. Information from studies of Drosophila fertility can then potentially be applied to understanding the genetics of human fertility.

描述（由申请人提供）：进化力对导致物种形成的遗传变异作用的过程仍然是生物学中最大的未解决问题之一。对物种形成过程的研究的两个主要目标是确定生殖障碍的基础变化，并确定推动其差异的进化力。为了检验有关物种物种的多种假设，首先识别负责物种差异的基因至关重要，这些基因目前非常有限，因为仅确定了四个影响生殖隔离的基因。该提案的重点是使用基因表达分析和转基因学来确定1）行为隔离的遗传基础，通过表征候选基因的表征，以防止成功进行交配，这些基因先前通过定量性状基因座映射，影响果蝇simulans和D. Mauritiana和D. Mauritiana和2）识别属于SIMITIL STERICE的基因的同胞在d.毛里蒂亚纳和D. sechellia。然后，来自果蝇生育能力的研究的信息可能会应用于理解人类生育能力的遗传学。