Selective Breeding for Ethanol Sensitivity

乙醇敏感性的选择性育种

• 批准号: 6966939
• 负责人: RICHARD A DEITRICH
• 金额: $ 22.9万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-18 至 2008-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6966939
• 关键词: Selective; Breeding; Ethanol; Sensitivity

DESCRIPTION (provided by applicant): This is a supplemental application to take advantage of a highly significant Quantitative Trait Locus (QTL) on chromosome 1 for high dose ethanol sensitivity that we have discovered in the Inbred Alcohol non- Tolerant (IANT) and the inbred Alcohol Tolerant (IAT rats) in spite of the fact that these animals were selectively bred for low dose effects. This QTL is in the same general location as one for this phenotype in the inbred High Alcohol Sensitive (IHAS) and inbred Low Alcohol Sensitive (ILAS) rats, but is much more significant and has a much higher heritability. We will take advantage of this unique opportunity to add to our knowledge of ethanol sensitivity by construction of congenic strains for the QTL, narrowing the region of the QTL and comparing the results to the same region in the I HAS and I LAS rats. We are also constructing congenic strains from the I HAS and I LAS rats on chromosomes 2 and 5. The QTL on chromosome 1 in the IHAS and ILAS rats is much smaller than in the IANT and IAT rats. These studies are important since among the risk factors for alcoholism in humans is a low initial sensitivity and/or rapid development of tolerance to ethanol. We have two animal models of this behavior, the rats selectively bred for high dose sensitivity (the IHAS and ILAS) and animals bred for low dose sensitivity (the IAT and IANT)

描述（由申请人提供）：这是一份补充申请，旨在利用 1 号染色体上高度显着的数量性状基因座 (QTL)，以实现我们在近交酒精不耐受 (IANT) 和近交品种中发现的高剂量乙醇敏感性。酒精耐受性（IAT 大鼠），尽管这些动物是为了低剂量效应而选择性培育的。该 QTL 与近交高酒精敏感 (IHAS) 和近交低酒精敏感 (ILAS) 大鼠中该表型的 QTL 处于相同的位置，但更重要且具有更高的遗传力。我们将利用这个独特的机会，通过构建 QTL 的同源菌株、缩小 QTL 区域并将结果与​​ I HAS 和 I LAS 大鼠的相同区域进行比较，来增加我们对乙醇敏感性的了解。我们还在 2 号和 5 号染色体上构建 I HAS 和 I LAS 大鼠的同源品系。IHAS 和 ILAS 大鼠 1 号染色体上的 QTL 比 IANT 和 IAT 大鼠小得多。这些研究很重要，因为人类酗酒的危险因素之一是对乙醇的初始敏感性较低和/或快速发展的耐受性。我们有两种这种行为的动物模型，选择性饲养的高剂量敏感性大鼠（IHAS 和 ILAS）和低剂量敏感性饲养的动物（IAT 和 IANT）