ROLE OF ACETALDEHYDE IN CENTRAL ACTIONS OF ETHANOL

乙醛在乙醇的中心作用中的作用

• 批准号: 6261141
• 负责人: RICHARD A DEITRICH
• 金额: $ 9.55万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-10 至 2003-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6261141
• 关键词: acetaldehyde; acetates; alcohol dehydrogenase; alcoholic beverage consumption; animal breeding; behavior test; blood tests; carbon dioxide; catalase; central nervous system; cytochrome P450; drug metabolism; enzyme activity; enzyme inhibitors; enzyme mechanism; ethanol; gas chromatography; laboratory mouse; neurotoxicology; oxidation; pharmacokinetics; psychopharmacology; sleep; stoichiometry; substance abuse related behavior

DESCRIPTION: The first specific is designed to discover and identify the enzymes responsible for the oxidation of ethanol to acetaldehyde in the brains of mice. The second aim is to study the mechanisms by which the accumulation of acetaldehyde in the brain alters the effect of ethanol in the central nervous system. Preliminary results provide a correlation between ethanol induced hypnosis (sleep time) and brain acetaldehyde levels. It is a collaborative project between Dr. Sergey Zimatkin and his colleagues at the Institute of Biochemistry, Grodno, Belarus in the former Soviet Union and Dr. Richard Deitrich and his colleagues at the Alcohol Research Center at the University of Colorado Health Sciences Center. The Institute of Biochemistry in Grodno has long been known for its studies on the metabolism and actions of ethanol and have the expertise to carry out the biochemical studies necessary for this project. The animal breeding and behavioral tests will be conducted at the University of Colorado Alcohol Research Center. This Center has selectively bred mice for differential responses to ethanol and has a great deal of experience in the behavioral and genetic characterization of such animals. In addition, we now have acatalasemic mice as well as Cytochrome P450 2EI knockout mice. Since both catalase and this cytochrome have been implicated in ethanol oxidation, these knockout mice will be especially useful.

描述： 第一个具体目的是发现和识别酶 负责在大脑中将乙醇氧化成乙醛 老鼠。第二个目的是研究积累的机制。 大脑中的乙醛改变了乙醇对中枢神经的作用 系统。初步结果提供了乙醇诱导的 催眠（睡眠时间）和脑乙醛水平。这是一次协作 Sergey Zimatkin 博士和他在研究所的同事之间的项目 生物化学，前苏联白俄罗斯格罗德诺和理查德博士 戴特里希和他在大学酒精研究中心的同事 科罗拉多健康科学中心。格罗德诺生物化学研究所 长期以来因其对乙醇代谢和作用的研究而闻名 并拥有进行此所需的生化研究的专业知识 项目。动物饲养和行为测试将在 科罗拉多大学酒精研究中心。本中心有选择性地 培育出对乙醇有不同反应的小鼠，并且具有大量 此类动物的行为和遗传特征方面的经验。在 此外，我们现在有无过氧化酶小鼠以及细胞色素 P450 2EI 基因敲除小鼠。由于过氧化氢酶和这种细胞色素都与 乙醇氧化后，这些基因敲除小鼠将特别有用。