CORE--INTESTINAL MICROBIOLOGY

核心--肠道微生物学

• 批准号: 6564223
• 负责人: Andrew George Plaut
• 金额: $ 20万
• 依托单位: TUFTS MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-12-01 至 2002-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6564223
• 关键词: bacterial toxins; biological products; biomedical facility; enteric bacteria; fermentation; microorganism culture; microorganism mass culture; plasmids; protein purification; protozoa

DESCRIPTION: (Taken from the application) All the services to be provided by the Intestinal Microbiology Core are in some way related to the culture of microorganisms, since all our procedures either begin or end with the growth of a bacterium or parasite. The services have been designed to fill three different investigator needs: 1. Provide unique equipment and the skills needed to operate it, allowing investigators to carry out digestive disease-related research for which their own laboratories are not equipped. Examples are large volume culture of organisms requiring a BL2-large-scale level of containment, or culture of organisms that require specialized vessels, growth conditions, or special expertise (Giardia, Helicobacter). Another example is production of a protein whose purification requires not only large-scale cultures, but also safe and rapid reduction of 10-20 L of spent culture medium to manageable size, followed by protein purification. In most laboratories the safe handling of such volumes is extremely cumbersome and often not possible. 2. Perform procedures that various GRASP laboratories require only periodically, essential to their work but inefficiently done owing to infrequency of use. These procedures are within the capabilities of our users but are done in the core every day, with high consistency and quality. Examples of these services are production of competent cells and plasmid preparations. As revealed by the heavy use of these services, our scientists clearly find them valuable, cost-efficient, and a great convenience. 3. Perform services that are not complex, but time-consuming. This saves wear and tear on investigators and technicians, and saves their time. The best example in this category is media preparation.

描述：（摘自应用程序）提供的所有服务 肠道微生物学核心在某种程度上与文化有关 微生物，因为我们所有的程序都以微生物的生长开始或结束 细菌或寄生虫。这些服务旨在填补三个 不同的调查员需求： 1. 提供独特的设备和所需的技能 操作它，使研究人员能够进行与消化系统疾病相关的研究 他们自己的实验室没有配备的研究。例子很大 需要 BL2 大规模密封水平的生物体的体积培养， 或需要专门容器、生长条件的生物体培养，或 特殊专业知识（贾第虫、螺杆菌）。另一个例子是生产 蛋白质的纯化不仅需要大规模培养，还需要 安全快速地将 10-20 L 用过的培养基减少至可管理的大小， 然后进行蛋白质纯化。在大多数实验室中，安全处理 如此大的体积极其繁琐，而且往往是不可能的。 2. 执行 各种 GRASP 实验室仅定期要求的程序，必要的 他们的工作，但由于使用频率低而效率低下。这些 程序在我们用户的能力范围内，但在核心中完成 每一天，都具有高度的一致性和质量。这些服务的例子有 感受态细胞和质粒制剂的生产。据透露 大量使用这些服务，我们的科学家显然发现它们很有价值， 成本效益高，而且非常方便。 3. 执行不属于以下范围的服务 复杂，但耗时。这可以减少调查人员的磨损 技术人员，节省了他们的时间。此类别中最好的例子是媒体 准备。