Continuous DOT Imaging of Hemodynamics During Stroke

中风期间血流动力学的连续点成像

• 批准号: 6916303
• 负责人: JOSEPH P CULVER
• 金额: $ 14.62万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-07-03 至 2007-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6916303
• 关键词: analog; antihypercholesterolemic agent; autoradiography; bioimaging /biomedical imaging; biomedical equipment development; blood volume; brain circulation; brain disorder chemotherapy; brain imaging /visualization /scanning; brain metabolism; cerebral ischemia /hypoxia; combination chemotherapy; fibrinolysis; functional magnetic resonance imaging; hemodynamics; hypertension; laboratory rat; lovastatin; magnetic resonance imaging; neurophysiology; optical tomography; plasminogen activator; prognosis; reperfusion; somesthetic sensory cortex; stroke

DESCRIPTION (provided by applicant): Continuous DOT Imaging of Hemodynamics During Stroke" is submitted in response to the NINDS program announcement for Mentored Quantitative Research Career Awards (K25). The candidate seeks to gain training in the neurobiology of Stroke and to advance the methods of Diffuse Optical Tomography (DOT) for application in stroke physiology. Stroke is both caused by, and induces deranged hemodynamics. It is therefore not surprising that the hemodynamic status of ischemic tissue contains vital and abundant information about microvasculature integrity, deficient cerebral metabolism, and abnormal neuronal activity. Diffuse optical imaging of the brain is an attractive approach for evaluating stroke physiology. The method provides complementary hemodynamic and metabolic imaging contrasts to those of Magnetic Resonance Imaging (MRI) with a unique potential for continuous non-invasive bedside imaging in humans. However quantitative spatial-temporal, comparative studies with established medical imaging techniques are lacking. In addition, experience with diffuse optical signatures of stroke pathophysiology is limited, and mostly involves non-imaging measures. The goal of this work is to establish new DOT hemodynamic and metabolic methodologies through concurrent DOT-N4RI animal (rat) studies, and apply the methods to examine the spatial-temporal evolution of reperfusion and hemorrhagic transformation with thrombolytics. The new DOT techniques are based on NEU guided Monte-Carlo diffuse light modeling, and hybrid time resolved and continuous-wave light measurements. Methods for quantifying and imaging cerebral blood volume (CBV), cerebral blood flow (CBF), and indexes of cerebral oxygen metabolism (CMRO) will be validated through global and focal programmed modulations of cerebral vasculature and physiology. Subsequent studies will explore DOT markers relevant to focal ischemia, thrombolytics, and hemorrhage due to reperfusion. Through the training that this award provides the applicant will gain sufficient background in the neuroscience and cerebral ischemia to lead cross-disciplinary research projects that aim to further the knowledge of stroke physiology.

描述（由申请人提供）：中风期间血液动力学的连续点成像“是为了响应NINDS计划公告的公告，用于指导定量研究职业奖项（K25）。候选人试图在中风神经生物学方面获得培训，并促进了散布的光学症状（DOT），以促进diffuse smography（Dot），以实现Stremoge and Issoloke Issogy and Issoke Is is。因此，毫不奇怪，缺血组织的血液动力学状态包含有关微举行完整性，不足的大脑代谢和异常的神经元活性的重要信息。在人类中连续的非侵入性床边成像。此外，具有中风病理生理学弥漫性光学特征的经验有限，并且主要涉及非成像措施。这项工作的目的是通过并发的DOT-N4RI动物（大鼠）研究来建立新的DOT血液动力学和代谢方法，并采用研究再灌注和出血转化的空间 - 周期性演化的方法。新的DOT技术基于NEU引导的蒙特卡洛弥漫性光建模，并解决了混合时间和连续的波光测量。量化和成像脑血容量（CBV），脑血流量（CBF）和大脑氧代谢（CMRO）索引的方法将通过全球和局灶性程序化的脑血管和生理学调节验证。随后的研究将探索与局灶性缺血，血栓溶液和由于再灌注引起的出血有关的点标记。通过培训，该奖项提供的申请人将获得神经科学和脑缺血的足够背景，以领导跨学科研究项目，旨在进一步了解中风生理学的知识。