Function of group III mGluRs at the calyx of Held

Held 花萼第 III 族 mGluR 的功能

• 批准号: 6868901
• 负责人: ROBERT B RENDEN
• 金额: $ 4.83万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-03-01 至 2006-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6868901
• 关键词: auditory pathways; developmental neurobiology; electrophysiology; genetically modified animals; glutamate receptor; immunocytochemistry; laboratory mouse; laboratory rat; neural plasticity; neurogenesis; postdoctoral investigator; protein structure function; synapses; voltage /patch clamp

DESCRIPTION (provided by applicant): The medial nucleus of the trapezoid body is an essential sign-inverting synapse in the binaural sound localization circuit. The major synapse in the MNTB is the calyx of Held, a. glutamatergic axosomatic synapse, with a uniquely large presynaptic terminal. This morphology allows paired pre-and postsynaptic intracellular recordings, allowing real-time measurements of presynaptic vesicle release, and postsynaptic receptor responses. The calyx shows activity-dependent functional synaptic plasticity, and also undergoes well-characterized postembryonic developmental changes. There are several modulatory transmitters and receptors present at the calyx of Held, that likely play a role in shaping the synpatic properties during development. 0f these, the metabotropic glutamate receptors, (mGluRs) are enigmatic, as they do not to date show a well-defined physiological role in either synaptic plasticity, or synaptic development. My interest lies in investigating the functional role of the group Ill mGluRs, which are located presynaptically at the calyx of Held. Using a combination of immunohistochemistry, electrophysiology, and murine genetics, I will determine the identity and role of group Ill mGluRs in synaptic plasticity and development of the calyx.

描述（申请人提供）：梯形体的内侧核是双耳声音定位电路中重要的符号反转突触。 MNTB 中的主要突触是 Held 的花萼，a。谷氨酸轴体突触，具有独特的大突触前末端。这种形态允许配对的突触前和突触后细胞内记录，允许实时测量突触前囊泡释放和突触后受体反应。花萼显示出活动依赖性的功能突触可塑性，并且还经历了明显的胚胎后发育变化。 Held 的花萼处存在多种调节递质和受体，它们可能在发育过程中塑造突触特性方面发挥作用。除此之外，代谢型谷氨酸受体（mGluR）是神秘的，因为迄今为止它们在突触可塑性或突触发育中尚未显示出明确的生理作用。我的兴趣在于研究 Ill mGluR 组的功能作用，该组位于 Held 花萼的突触前。通过结合免疫组织化学、电生理学和小鼠遗传学，我将确定 III 组 mGluR 在突触可塑性和花萼发育中的身份和作用。