Development of Inhibition in the Avian Cochlear Nucleus

禽耳蜗核抑制的发展

• 批准号: 7113269
• 负责人: MacKenzie A Howard
• 金额: $ 3.24万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-01-09 至 2009-07-23
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7113269
• 关键词: GABA receptor; RNA interference; afferent nerve; auditory nuclei; auditory pathways; chick embryo; cochlear nerve; developmental neurobiology; electrical conductance; electrophysiology; electroporation; gamma aminobutyrate; genetic regulation; green fluorescent proteins; ion channel blocker; neural inhibition; neurogenesis; neurons; neurophysiology; nonmammalian vertebrate embryology; otocyst /otolith; phenotype; predoctoral investigator; sectioning; synapses; time perception; voltage /patch clamp

DESCRIPTION (provided by applicant): The auditory system localizes sound in azimuth by computing interaural time delays. In the avian auditory brainstem, nucleus magnocellularis (NM) is the first central nucleus in this temporal coding pathway. NM contains highly specialized anatomic, synaptic, and intrinsic properties that play key roles in shaping inhibitory responses and maintaining temporal fidelity in NM. The development of these specializations is poorly understood. The first aim is to describe the time-course of the development of inhibition in chick NM using in vitro electrophysiological techniques.. The second aim is to understand the developmental importance of afferent excitatory inputs in the development of inhibition using otocyst removal followed by physiological recordings. The third aim will examine role of Kv1.1 in developing the normal NM phenotype using RNA interference techniques. Plasmids encoding RNAs designed to interfere with the Kcnal, the Kv1.1 gene, and an EGFP reporter gene will be electroporated in ovo in early embryos. The physiology of transfected cells will be examined through development. These manipulations are hypothesized to alter the normal development of NM anatomy and physiological responses to excitatory and inhibitory inputs.

描述（由申请人提供）：听觉系统通过计算播出时间延迟来将声音定位在方位角中。在禽听觉的脑干中，核细胞核（NM）是该时间编码途径中的第一个中央核。 NM包含高度专业化的解剖，突触和内在特性，这些特性在塑造抑制反应和保持NM中的时间忠诚度中起着关键作用。这些专业的发展知之甚少。第一个目的是使用体外电生理技术来描述小鸡NM抑制作用的时间顺序。第二个目的是了解传入兴奋性投入在使用耳尾抑制抑制过程中使用耳尾去除的发展重要性，然后再进行生理记录。第三个目标将检查KV1.1在使用RNA干扰技术开发正常NM表型中的作用。编码用于干扰KCNAL，KV1.1基因和EGFP报告基因基因的RNA的质粒将在早期胚胎中的OVO中进行电穿孔。转染细胞的生理学将通过发育检查。假设这些操纵以改变NM解剖结构的正常发展以及对兴奋性和抑制性投入的生理反应。