Core--Mass Spectrometry

核心--质谱

• 批准号: 6725957
• 负责人: RICHARD M CAPRIOLI
• 金额: $ 18.46万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-01 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6725957
• 关键词: DNA damage; analytical chemistry; biomedical equipment development; biomedical equipment resource; biomedical facility; environmental toxicology; mass spectrometry; protein structure function

Description (provided by applicant): The mission of the MS Core is to provide cost effective, state-of-the-art instrumentation and analytical expertise to investigators in the Center for Molecular Toxicology for identification of protein and DNA adducts, biophysical and structural analysis of proteins, and for qualitative and quantitative assays of metabolites in tissues and cells. The MS Core is used extensively by eleven of the Center in Molecular Toxicology investigators. Laboratory personnel assist users in developing analytical methods, assist in experimental design, develop standard operating procedures, maintain quality control records on instrument performance and maintenance history, perform routine assays for investigators and train students and fellows in the theoretical and practical aspects of MS. The Core is run as an open-access facility in which users are expected to prepare their own samples and to operate the instruments if they so desire. Administrative staff monitors the use of the instrument facilities by investigators and prepares reports on utilization. The Core has five triple quadrupole liquid chromatography (LC)/MS instruments, one LC/MS ion trap instrument, an electrospray ionization time-of-flight MS (ESI-TOF), two GC/MS systems, and a matrix-assisted laser desorption ionization (MALDI) mass spectrometer. The MS Core is part of the Vanderbilt University Mass Spectrometry Research Center, a comprehensive research unit located in the Medical School. This facility consists of a Core MS service laboratory and the Proteomics Research Core. In addition to providing routine analytical and MS services via the various Core facilities, investigators can form collaborations to solve analytical and structural problems that require non-routine, cutting edge technology. As newer techniques become better understood, they will be made available to the user community through the Core service laboratory. Thus, the Core satisfies the routine analytical needs of investigators and also makes available new analytical capabilities for research problems demanding more innovative solutions that are beyond the scope of a service laboratory. In addition, a training program on various aspects of MS is offered so users can learn to operate the instruments, design experiments, and interpret analytical results more effectively. Both the Core and Research laboratories were extensively renovated in 1998 and new instrumentation was purchased to provide a state-of-the-art facility. Support for the facility comes from service cores affiliated with eight NIH-funded Program Projects and Research Centers, service fees charged to unaffiliated investigators and direct institutional support form Vanderbilt University.

描述（由申请人提供）：MS Core的使命是为分子毒理学中心的研究者提供具有成本效益的，最先进的仪器和分析专业知识，以鉴定蛋白质和DNA加合物，蛋白质的生物物理和结构分析，蛋白质的生物物理和结构性分析，以及用于组织和细胞中代谢物的定性和定量分析的蛋白质和定性和定量分析。 MS核心在分子毒理学研究者中被11个中心广泛使用。实验室人员协助用户开发分析方法，协助实验设计，制定标准操作程序，维护仪器性能和维护历史记录的质量控制记录，对MS的理论和实用方面的调查人员和培训学生和研究员进行例行测定。该核心是作为开放式设施运行的，希望用户可以根据需要准备自己的样品并操作仪器。行政人员监视调查人员使用仪器设施的使用，并准备有关利用的报告。该核心具有五个三倍四极液色谱法（LC）/MS仪器，一种LC/MS离子陷阱仪器，电喷雾电离飞行时间MS（ESI-TOF），两个GC/MS系统和一个基质辅助激光剂降压电离电离（MALDI）质谱仪。 MS Core是范德比尔特大学质谱研究中心的一部分，该研究中心位于医学院。该设施由MS服务实验室和蛋白质组学研究核心组成。除了通过各种核心设施提供常规的分析和MS服务外，研究人员还可以进行协作以解决需要非列场，最先进技术的分析和结构性问题。随着更新的技术变得更好地理解，它们将通过核心服务实验室提供给用户社区。因此，核心满足了调查人员的常规分析需求，并为研究问题提供了新的分析能力，要求需要更具创新的解决方案，这些解决方案超出了服务实验室的范围。此外，还提供了有关MS各个方面的培训计划，以便用户可以学习操作工具，设计实验并更有效地解释分析结果。 核心和研究实验室在1998年进行了广泛翻新，并购买了新的仪器以提供最先进的设施。 对该设施的支持来自由八个由NIH资助的计划项目和研究中心的服务核心，收取的服务费以及范德比尔特大学的直接机构支持。