New Mexico Center for Metals in Biology and Medicine - Equipment Supplement

新墨西哥生物和医学金属中心 - 设备补充

• 批准号: 10395875
• 负责人: Matthew J Campen
• 金额: $ 25万
• 依托单位: UNIVERSITY OF NEW MEXICO HEALTH SCIS CTR
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-07-01 至 2025-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10395875
• 关键词: Analytical Chemistry; Binding; Biological; Biological Assay; Biological Markers; Biology; Biomedical Research; Clinical; Communities; Complement; Core Facility; DNA Damage; Development; Equipment; Funding; Health; Health Sciences; High Prevalence; Injections; Intervention; Knowledge; Laboratories; Lead; Medicine; Metals; Methods; Mining; Molecular Analysis; National Institute of General Medical Sciences; New Mexico; Nutritional; Outcome; Peptides; Population; Productivity; Protein Analysis; Public Health; Research; Resources; Sampling; System; Techniques; Therapeutic; Universities; base; chemical property; cost effective; equipment acquisition; improved; innovation; instrument; instrumentation; interest; lipidomics; member; metabolomics; metallicity; small molecule

Administrative Suppl. for Equipment Purchases for NIGMS-funded Center and Core Facilities NOT-GM-21-029 Summary Inorganic metals have long been a focus of biomedical research in New Mexico, due to the high prevalence of mining-related metal contaminants in the region. At the University of New Mexico Center for Metals in Biology and Medicine (UNM CMBM), our main focus is on both studying adverse health outcomes of metal contaminants as well as on harnessing the chemical properties of metals for therapeutic and nutritional purposes. Detailed knowledge of molecular interactions between metallic species and biomolecules can lead to clinical and population-level interventions to improve public health. Towards this end, we have established a new Integrative Molecular Analysis Core (IMAC) to provide members, and the UNM Health Sciences Center campus, with cutting edge analytical chemistry instrumentation and techniques. is launching, still in its first year, and seeing a surge of user interest and activity. In responding to needs of our research community, several specific analytical applications require additional specialized instrumentation for high-accuracy small molecule quantitation have been discussed. To provide the membership with a workhorse machine that can handle targeted metabolomic and lipidomic assays, as well as examine DNA damage, oxidative biomarkers, and metal-associated biomolecules, the Sciex 5500+ Triple QuadTM offers a superb cost-effective system to meet our diverse quantitation needs. Featuring a high level of sensitivity, selectivity and linear dynamic range, it allows for the most demanding quantitation analyses, such as challenges of severely interfered low-level trace quantification that are commonly encountered during analysis of specific metabolites and biomarkers in biological and environmental samples. Triple Quad LC-MS/MS systems deliver superior quantitative results in a single injection workflow. By offering robust targeted quantitative analysis of known small molecules such as metabolites, biomarkers and peptides of interests, it is a highly beneficial addition and expansion to our Orbitrap LC-HRMS platform that is preferentially used for non-targeted protein analysis. This state-of-the-art system is the first of its kind on our HSC campus. It will complement our current MS systems to provide new and extended applications for our user base. Moreover, faster and more sensitive instrument will help with our constant development of innovative bioanalytical methods to meet new research needs of our users. Establishing this capacity at UNM will certainly meet the needs of a wider audience and expand the value of our IMAC for the UNM HSC. Therefore, the 5500+ system will enhance our user access to and impact of the resource, improve the productivity and efficiency in the laboratory, and help sustain the Core.

行政补充。用于NIGMS资助的中心和核心设施的设备购买 总结无机金属长期以来一直是新墨西哥州生物医学研究的重点 该地区与采矿相关的金属污染物的患病率。在新墨西哥大学 生物学和医学金属（UNM CMBM），我们的主要重点是研究不良健康结果 金属污染物以及利用金属的化学特性用于治疗和营养 目的。金属物种与生物分子之间分子相互作用的详细知识可以领导 进行临床和人群水平的干预措施，以改善公共卫生。为此，我们已经建立了 新的综合分子分析核心（IMAC）提供成员和UNM健康科学中心 校园，具有最先进的分析化学仪器和技术。正在发射，仍然处于第一个 一年，看到用户的兴趣和活动激增。在响应我们的研究社区的需求时， 几种特定的分析应用需要其他专业仪器，以便高临界性小 已经讨论了分子定量。为会员提供可以使用的机器机器 处理靶向的代谢组学和脂肪组分析，并检查DNA损伤，氧化生物标志物， 和与金属相关的生物分子，Sciex 5500+三倍QUADTM为 满足我们多样化的定量需求。具有高度的灵敏度，选择性和线性动态范围， 它允许进行最苛刻的定量分析，例如严重干扰低级的挑战 在分析特定代谢物和生物标志物中通常遇到的痕量定量 生物和环境样品。三重四分之一LC-MS/MS系统在 单个注入工作流程。通过对已知的小分子（例如 代谢产物，生物标志物和感兴趣的肽是对我们的非常有益的补充和扩展 优先用于非靶向蛋白质分析的Orbitrap LC-HRMS平台。这个最新的 系统是我们HSC校园中的第一个。它将补充我们当前的MS系统以提供新的 并为我们的用户群扩展了应用程序。而且，更快，更敏感的工具将有助于我们 不断开发创新的生物分析方法，以满足用户的新研究需求。 在UNM中建立这种能力肯定会满足更广泛的受众的需求，并扩大 我们的iMac用于UNM HSC。因此，5500+系统将增强我们对用户的访问和影响 资源，提高实验室的生产率和效率，并帮助维持核心。