Development of Optofluidic Resonators for Filoviral Detection

用于丝状病毒检测的光流控谐振器的开发

• 批准号: 10506889
• 负责人: Abraham Jaleel Qavi
• 金额: $ 7.31万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-08-01 至 2023-01-06
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10506889
• 关键词: 2019-nCoV; Address; Affinity; Africa; Africa South of the Sahara; Area; Binding; Biochemistry; Biological Assay; Biophysics; Biosensing Techniques; Buffers; COVID-19 pandemic; California; Case Fatality Rates; Cessation of life; Chemistry; Clinical Pathology; Cold Chains; Communicable Diseases; Complex; Coupling; Data; Democratic Republic of the Congo; Detection; Development; Development Plans; Devices; Diagnostic; Disease; Disease Outbreaks; Doctor of Philosophy; Ebola; Ebola Hemorrhagic Fever; Ebola virus; Ebola virus envelope glycoprotein; Electronics; Engineering; Enrollment; Enzyme-Linked Immunosorbent Assay; Filovirus; Foundations; Glycoproteins; Goals; Hospitals; Illinois; Immune response; Immunology; Individual; Infection; International; Laboratories; Lasers; Lassa virus; Lead; Light; Liquid substance; Measurement; Medical; Mentors; Mentorship; Methods; Microbubbles; Molecular Biology; Monoclonal Antibodies; NCI Scholars Program; Optics; Output; Pathogen detection; Pathogenicity; Pathology; Pathway interactions; Performance; Phase; Physicians; Positioning Attribute; Process; Protein Biochemistry; Protein Engineering; Proteins; RNA Viruses; Reagent; Reporter; Research; Research Personnel; Residencies; Sampling; Scientist; Sentinel; Serology; Signal Transduction; Source; System; Techniques; Testing; Time; Training; Training Programs; Universities; Validation; Vesicular stomatitis Indiana virus; Viral; Viral Genome; Viral Hemorrhagic Fevers; Viral Markers; Virus; Virus Diseases; Washington; Work; Yang; analytical tool; antibody engineering; antibody mimetics; base; career; career development; cost; design; detection method; diagnostic assay; emerging pathogen; follower of religion Jewish; human pathogen; multiplex detection; nanoparticle; novel; novel diagnostics; optical sensor; particle; pathogen; photonics; professor; programs; rapid detection; response; sensor; sensor technology; standard of care; training opportunity; viral detection; virology; virus envelope

Project Summary/Abstract This proposal describes a 4-year career development plan for the PI, Dr. Abraham J. Qavi, MD, PhD, with the goal of preparing him for an independent research career as a physician scientist. This plan includes expand training opportunities and a pathway to an independent career that includes the development and implementation of sensor technologies and its application towards filoviruses, namely Ebola. The PI graduated with degrees in Biochemistry & Molecular Biology and Chemistry from the University of California, Irvine. He then enrolled in the Medical Scholars Program at the University of Illinois at Urbana-Champaign, where he earned his MD and PhD in Chemistry. Dr. Qavi continued his medical training as part of the Clinical Pathology Physician Scientist Training Program at Washington University in St. Louis and the Barnes-Jewish Hospital consortium. During his residency elective time, he began research in the laboratory of Dr. Lan Yang, who will serve as his co-mentor together with Dr. Amarasinghe. Dr. Yang is the Edwin H. and Florence G. Skinner Professor of Electrical & Systems Engineering, and an internationally renowned researcher in photonics. In 2019, Dr. Qavi added an additional training component under the co-mentorship with Dr. Gaya Amarasinghe, Professor of Pathology & Immunology, to expand the application of his previously developed sensor technology to infectious disease reach. The goal of this study is the development of a rapid, multiplexed optofluidic sensor platform for the rapid detection of pathogens, with an initial focus on Ebola. Filoviruses, such as Ebola, are among the most lethal human pathogens, with high case fatality rates during outbreaks. Critical in the identification and management of outbreaks are robust detection methods that can be implemented rapidly and sensitively. To address this critical need, Whispering Gallery Mode (WGM) sensors will be leveraged. WGM devices are a class of optical sensors in which light is confined within a micron-scale volume. These devices have incredibly high sensitivity, small sensor footprint, ease of integration with conventional electronics, and low fabrication costs. Microbubble resonators (MBRs), a subclass of WGM sensors, offer the advantages of conventional WGM devices while enabling coupling of optical and the fluidic components into a single component. The goal of this proposal is the use of an optofluidic sensor platform based on MBRs for the rapid, multiplexed detection of Ebola. The workflow and advancements, including MBR development, engineered antibodies, biophysical validation and multiplexing, will provide the framework to extend the work beyond the initial goals and promote facile transition to an independent career for Dr. Qavi.

项目摘要/摘要 该建议描述了PI的4年职业发展计划，Abraham J. Qavi博士，博士，博士 为他作为医生科学家的独立研究生涯做好准备。该计划包括扩展 培训机会和通往独立职业的途径，包括开发和实施 传感器技术及其对丝状病毒的应用，即埃博拉病毒。 PI毕业于学位 加利福尼亚大学尔湾分校的生物化学与分子生物学与化学。然后他加入了 伊利诺伊大学Urbana-Champaign大学的医学学者计划，在那里他获得了医学博士学位和博士学位 在化学中。 Qavi博士继续接受医学培训，作为临床病理医师科学家培训的一部分 在圣路易斯华盛顿大学和巴恩斯 - 犹太医院财团的计划。在他的居住期间 选举时间，他开始在Lan Yang博士的实验室进行研究，Lan Yang博士将担任他的同事 Amarasinghe博士。 Yang博士是Edwin H.和Florence G. Skinner电气与系统教授 工程学，以及国际著名的光子学研究员。 2019年，Qavi博士增加了一份 与病理学和免疫学教授Gaya Amarasinghe博士的培训组成部分 扩大他先前开发的传感器技术在传染病范围内的应用。 这项研究的目的是开发快速，多路复用的光流传感器平台 病原体的快速检测，最初侧重于埃博拉病原体。埃博拉病毒（例如埃博拉病毒）是 大多数致命的人类病原体，暴发期间病例死亡率很高。对识别至关重要 爆发的管理是可迅速和敏感的强大检测方法。到 解决这种关键需求，将利用耳语画廊模式（WGM）传感器。 WGM设备是一类 光传感器的光限制在微米尺度内。这些设备的高度很高 灵敏度，小型传感器足迹，与常规电子设备的易于集成以及低制造成本。 WGM传感器子类的微气泡谐振器（MBRS）提供了常规WGM的优势 设备将光学和流体组件耦合到单个组件中。目标的目标 提案是基于MBR的Optofluidic传感器平台的使用，用于快速，多路复用的埃博拉病毒。 工作流程和进步，包括MBR开发，工程抗体，生物物理验证 和多路复用，将提供框架以扩展工作超出初始目标并提升便利 过渡到Qavi博士的独立职业。