Biomolecular Multimodal Imaging Center: 3-Dimensional Tissue Mapping of the Human Pancreas and Eye

生物分子多模态成像中心：人类胰腺和眼睛的 3 维组织图谱

• 批准号: 10254369
• 负责人: RICHARD M CAPRIOLI
• 金额: $ 96.18万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-30 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10254369
• 关键词: 3-Dimensional; Academic Medical Centers; Alabama; Applications Grants; Atlases; Cells; Clinical; Clinical Data; Collaborations; Collection; Communities; Data; Data Analyses; Data Set; Detection; Development; Diabetes Mellitus; Dimensions; Disease; Ensure; Environment; Excision; Eye; Florida; Foundations; Freezing; Funding; Future; Generations; Health; Histologic; Histology; Human; Human BioMolecular Atlas Program; Image; Imaging technology; Immunofluorescence Immunologic; Infrastructure; Kidney; Laboratories; Lipids; Mass Spectrum Analysis; Medical Imaging; Medical center; Microscopy; Mission; Modality; Molecular; Molecular Analysis; Monitor; Multimodal Imaging; Netherlands; Normal tissue morphology; Operative Surgical Procedures; Optical Coherence Tomography; Organ; Organ Procurements; Pancreas; Participant; Performance; Physicians; Positioning Attribute; Preparation; Procedures; Process; Proteins; Proteomics; Quality Control; Reproducibility; Research; Research Personnel; Resources; Sampling; Scientist; Source; Specimen; Stains; Standardization; Surface; System; Technical Expertise; Techniques; Technology; Testing; Three-Dimensional Imaging; Tissues; United States National Institutes of Health; Universities; body system; cell type; chemical fixation; computing resources; data acquisition; ex vivo imaging; experience; human tissue; imaging modality; in vivo; indexing; innovation; insight; member; metabolomics; multidisciplinary; multimodality; preservation; programs; quality assurance; single-cell RNA sequencing

PROJECT SUMMARY – Overall. This application proposes the founding of a Tissue Mapping Center (TMC) for the human eye and pancreas within the Human BioMolecular Atlas Program (HuBMAP). The mission of the proposed TMC is to build a platform of integrated technologies for imaging and molecular analysis that enables the construction of comprehensive 3-dimensional molecular atlases of human eye and pancreas. This TMC will leverage the unique resources of the Mass Spectrometry Research Center and the National Research Resource for Imaging Mass Spectrometry at Vanderbilt University, the world-class clinical environment of the Vanderbilt University Medical Center, the distinguished expertise of Organ Specific Project team leaders in their respective fields and their vast experience in organ procurement and management, and the advanced biocomputational infrastructure available to the TMC through the Data Analysis Core laboratories at Vanderbilt University and the Delft University of Technology, Netherlands to create a capability to molecularly characterize human tissues in 3-dimensions at a level of understanding unrivaled by current technologies. The innovative aspects of the proposed TMC are (1) the integration of imaging mass spectrometry, co-detection by indexing (CODEX), stained microscopy, and autofluorescence microscopy into 3-dimensional molecular atlases, (2) whole organ imaging of the eye, and (3) multi-organ atlases from the same donors through a proposed collaboration with the currently funded University of Florida TMC. The application of the proposed platform to organ-specific projects in pancreas and eye will provide a new paradigm of understanding the normal state of these organs across vast scales, both molecular (e.g., lipids, metabolites, and proteins) and spatial (e.g., whole organs to single cells). Furthermore, the involvement of these organs in diabetes, along with organs currently funded by HuBMAP such as kidney and vasculature, will lay an important foundation for future studies striving to understand the progression of diabetes. As a HuBMAP participant, the molecular atlases produced by this TMC will be disseminated to collaborators to generate new hypotheses regarding the function of these important organ systems, enabling new insight into human health and disease. This multidisciplinary effort requires the creation and integration of three capabilities to 1) procure and manage human tissue specimens, 2) determine and mitigate non-biological pre-analytical factors, and 3) acquire, process, and disseminate multimodal 3-dimensional imaging and large-scale omics data.

项目摘要 - 总体。该应用建议建立组织映射中心（TMC） 对于人类生物分子图书馆（Hubmap）中的人眼和胰腺。任务 提出的TMC是建立一个集成技术的平台，用于成像和分子分析，以实现 人眼和胰腺的综合3维分子图谱的构建。这个TMC会 利用质谱研究中心和国家研究的独特资源 范德比尔特大学（Vanderbilt University）成像质谱的资源，世界一流的临床环境 范德比尔特大学医学中心，特定器官项目团队领导者的杰出专业知识 他们各自的领域以及他们在器官采购和管理方面的丰富经验，以及高级 TMC可通过范德比尔特的数据分析核心实验室可用于TMC的生物计算基础设施 荷兰大学和代尔夫特技术大学，以创造出分子的能力 在三维中表征人类组织，在当前技术无与伦比的理解水平上。这 提出的TMC的创新方面是（1）成像质谱的整合，通过 索引（法典），染色显微镜和自动荧光显微镜成3维分子 地图酶，（2）眼睛的整个器官成像，以及（3）通过同一供体的多器官地图 拟议与目前资助的佛罗里达大学TMC合作。提议的应用 胰腺和眼睛特定器官项目的平台将提供一个新的理解范式 这些器官的正常状态跨分子（例如脂质，代谢物和蛋白质）和 空间（例如，整个器官与单个细胞）。此外，这些器官参与糖尿病 凭借目前由肾脏和脉管系统等Hubmap资助的器官，将为 未来的研究努力了解糖尿病的进展。作为Hubmap参与者，分子 该TMC生产的地图集将被传播给合作者，以产生有关有关的新假设 这些重要的器官系统的功能，使人们可以对人类健康和疾病进行新的见解。这 多学科的努力需要创建和集成三个功能到1）采购和管理 人体组织标本，2）确定并减轻非生物的分析前因子，3）获取， 过程，并传播多模式3维成像和大规模的OMICS数据。