CORE--Cellular and Molecular Physiology Core

CORE--细胞和分子生理学核心

• 批准号: 6797505
• 负责人: JAMES Lorenzen BOYER
• 金额: $ 23.13万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-01 至 2009-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6797505
• 关键词: biomedical facility; gene expression; isolation perfusion; liver cells; polymerase chain reaction; tissue /cell culture; tissue /cell preparation; transfection

This Core facility is organized to provide technical expertise, equipment and personnel to Liver Center Investigators who wish to work with: a) Isolated cell preparations including: hepatocytes, cholangiocytes, endothelial cells, stellate cells and hepatic lymphocytes, primarily from rat and mouse b) Cell culture facilities for short and long term cultures and cell lines c) Isolated liver perfusion preparations for studies utilizing the whole organ d gene expression using Quantitative real time PCR machine and Phosphor-imaging equipment or e) Alter expression of genes in these cells and tissues using is RNA and adenovirus transfection technologies. By centralizing these procedures in a Core facility, investigators are assured of a high degree of quality control and preparations can often be used simultaneously by more then one investigator, e) Services a-c are also established at the Center?s satellite facility at the Mount Desert Island Biological Laboratory for use of alternative models in marine species, primarily the small skate, Raja erinacea.

该核心设施旨在为希望研究以下内容的肝脏中心研究人员提供技术专业知识、设备和人员： a) 分离的细胞制剂，包括：肝细胞、 胆管细胞、内皮细胞、星状细胞和肝淋巴细胞，主要来自大鼠和小鼠 b) 用于短期和长期培养和细胞系的细胞培养设施 c) 用于利用整个器官进行研究的离体肝脏灌注制剂 d 使用定量实时 PCR 进行基因表达机器和荧光成像设备或 e) 使用 RNA 和腺病毒转染技术改变这些细胞和组织中的基因表达。通过将这些程序集中在核心设施中，研究人员可以确保高度的质量控制，并且准备工作通常可以由多名研究人员同时使用，e) 服务 a-c 也在沙漠山的中心卫星设施中建立岛屿生物实验室在海洋物种（主要是小鳐鱼、Raja erinacea）中使用替代模型。