Strial vascular pathology from acoustic trauma

声损伤引起的心房血管病理学

• 批准号: 10174903
• 负责人: Xiaorui Shi
• 金额: $ 39.16万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-07-01 至 2023-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10174903
• 关键词: 3-Dimensional; Acoustic Trauma; Affect; Afferent Neurons; Aging; Animals; Auditory; Auditory Threshold; Basement membrane; Biological; Blood; Blood Vessels; Blood capillaries; Blood flow; Brain; Bromodeoxyuridine; Caliber; Cardiology; Cell Line; Cells; Clinical; Cochlea; Communication; Confocal Microscopy; Consumption; Development; Disease; Ear; Edema; Electron Microscopy; Endothelial Cells; Energy Supply; Exposure to; Extravasation; Failure; Foundations; Functional disorder; Gene Delivery; Goals; Growth Factor; Hair Cells; Health; Hearing; Hearing problem; Heart; Hormones; Human; Hypoxia; Immunophenotyping; Impairment; In Vitro; Individual; Infarction; Injury; Kidney Diseases; Label; Labyrinth; Lateral; Lead; Life; Ligands; Loudness; Maintenance; Mediating; Membrane Proteins; Mesenchymal; Metabolic; Modeling; Molecular; Mus; Myocardial Infarction; Myofibroblast; Natural regeneration; Neurons; Noise; Nuclear; Organ; PDGFA gene; Pathologic; Pathology; Pericytes; Phenotype; Physiology; Platelet-Derived Growth Factor B; Platelet-Derived Growth Factor beta Receptor; Population; Production; Proliferating; Property; Proteins; Recovery; Regulation; Reporter; Research; Residual state; Resolution; Retina; Role; Sensory; Signal Pathway; Signal Transduction; Site; Social isolation; Source; Stress; Stria Vascularis; Stroke; Structure; Sudden Deafness; System; Testing; Tissues; Transforming Growth Factor beta; Transforming Growth Factors; Transgenic Mice; Transplantation; Trauma; Vascular Diseases; Vascular blood supply; angiogenesis; base; blood perfusion; deafness; density; diabetic patient; feasibility testing; fibrogenesis; hearing impairment; hearing preservation; heart function; improved; inhibitor/antagonist; injury and repair; matrigel; migration; neonatal mice; network models; neurotrophic factor; novel; novel strategies; pigment epithelium-derived factor; platelet-derived growth factor BB; preservation; prevent; protein expression; receptor; repaired; restoration; sound; stem cells; success; therapeutic target; wound healing

PROJECT SUMMARY Energy supply to the ear is critical for hearing function since the ear is one of the highest energy consuming organs. Insufficient energy can result from insufficient blood flow to the cochlea contributing to a wide range of clinical hearing disorders such as loud sound-induced hearing loss, hearing loss related to ageing, and sudden deafness, which can largely impact the quality of human life by causing individual communication problems and social isolation. We believe that success in repair and regeneration of hearing function following loss of sensory cells requires parallel restoration or maintenance of an efficient blood supply. The proposed research is part of a longer range study on the role of pericytes in the physiology of the cochlea, but is specifically focused on the pericyte pathology that occurs in loud sound-induced lateral wall microcirculatory dysfunction. Pericytes are multipotent mesenchymal-like cells and are primarily located on microvessels. Normal function of pericytes is vital for blood flow regulation, vascular integrity, angiogenesis and tissue fibrogenesis. Pericyte pathology is profoundly associated with many organ diseases such as brain stroke, heart infarction, and retinal failure. Therapeutic targeting of pericytes has been considered a novel treatment for many of those clinical diseases. Cochlear pericytes are extremely vulnerable and sensitive to damage, but are critical for regulation of cochlear blood flow and maintaining tightness of the blood-labyrinth barrier in the stria vascularis. More specifically they are highly responsive to stress such as acoustic trauma. Upon exposure to loud sound, cochlear pericytes undergo striking changes in their biological properties, but the molecular mechanisms that underline those changes have not yet been studied. In this five year proposal, we will determine what molecular signals lead to loud sound-induced pericyte migration away from the capillaries and their phenotype changes. We will also determine whether transplantation of fresh pericytes such as neo-pericytes (derived from neonatal mice) to noise-damaged cochlea can repair loud sound-damaged microvessels and restore vascular function. The success of each aim will inevitably lead to the development of new protective and restorative therapies for a normal blood flow to cochlea― the critical foundation of hearing preservation or/and restoration.

项目概要 耳朵的能量供应对于听力功能至关重要，因为耳朵是能量消耗最高的部位之一 耳蜗血流不足可能导致能量不足，从而导致多种疾病。 临床听力障碍，例如大声引起的听力损失、与衰老相关的听力损失以及突发性听力损失 耳聋，会导致个人沟通问题，从而在很大程度上影响人类的生活质量 我们相信，听力损失后听力功能的修复和再生会取得成功。 感觉细胞需要并行恢复或维持有效的血液供应。 是关于周细胞在耳蜗生理学中的作用的长期研究的一部分，但具体是 专注于大声声音引起的侧壁微循环功能障碍中发生的周细胞病理学。 周细胞是多能间充质样细胞，主要位于微血管上，具有正常功能。 周细胞对于血流调节、血管完整性、血管生成和组织纤维生成至关重要。 病理学与许多器官疾病密切相关，例如脑中风、心脏病和视网膜疾病 针对周细胞的治疗已被认为是许多临床治疗的新方法。 耳蜗周细胞极其脆弱且对损伤敏感，但对于调节至关重要。 耳蜗血流并维持血管纹中血迷路屏障的紧密性。 特别是，当暴露于大声的声音时，它们对压力（例如声损伤）高度敏感。 耳蜗周细胞的生物学特性经历了显着的变化，但其分子机制 强调那些尚未研究的变化。在这个五年提案中，我们将确定哪些内容。 分子信号导致大声的声音诱导周细胞迁移远离毛细血管及其表型 我们还将确定是否移植新鲜周细胞，例如新周细胞（衍生自）。 新生小鼠）对噪声损伤的耳蜗进行修复可以修复因大声声音损伤的微血管并恢复血管 每个目标的成功将不可避免地导致新的保护和恢复功能的发展。 耳蜗正常血流的治疗——听力保留或/和恢复的关键基础。