Hybrid Nuclear/X-ray Projection System for Mouse Imaging

用于小鼠成像的混合核/X 射线投影系统

• 批准号: 6848595
• 负责人: JOHN M BOONE
• 金额: $ 23.87万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-24 至 2006-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6848595
• 关键词: X ray; bioengineering /biomedical engineering; bioimaging /biomedical imaging; biological models; biomedical equipment development; computer program /software; computer simulation; computer system design /evaluation; computer system hardware; gamma radiation; image processing; laboratory mouse; neoplasm /cancer; phantom model; printing; radiation dosage; radiography; radionuclide imaging /scanning; radionuclides

DESCRIPTION (provided by applicant): The mouse has become the mainstay of genetic and cancer research, and mouse imaging has allowed scientists to monitor tumor development or remission serially. The combination of mu/PET, which provides functional biological information (e.g. tumor uptake, gene expression, etc.) with mu/CT techniques, which provides the anatomical context for the functional information, has proved to be a very important tool for this. However, the radiation dose associated with these procedures can be high, and this limits their frequent use. While the radiation does not harm the animal, it may affect immune response mechanisms and change the experimental outcome. We hypothesize that 2D images may be sufficient in some scientific applications to provide the biological information necessary, in lieu of 3D image data sets that mu/CT and mu/PET provide. In this application, we propose the development of a hybrid two-dimensional imaging system for the mouse and other small animals. This system uses 7-ray projection imaging combined with x-ray projection radiography, and we refer to this as MINX (mouse imaging using nuclides and x-rays). The MINX system uses a single computed radiography CR detector (BaFBr imaging plate) to capture both the 7-ray and x-ray images. We hypothesize that the MINX system will provide mouse researchers with information similar to mu/PET/mu/CT, however the reduced radiation dose and lower imaging costs will allow investigators to perform more imaging on the same animal and therefore collect serial data more frequently. The six Specific Aims include (1) Monte Carlo and numerical simulation studies to define the most optimal MINX system design, (2) the manufacture of a parallel hole collimator appropriate for mouse imaging using microlithography techniques, (3) the construction of the MINX system itself, (4) the development of appropriate phantoms followed by phantom imaging, (5) initial studies on mice for further optimization, and finally (6) serial analysis using the MINX system on mice simultaneous with mu/CT/mu/PET imaging for comparison. This study is designed to demonstrate the utility of fiducially aligned 2-D nuclear/x-ray projection images in mouse research, and we expect that the development of the MINX system will give mouse researchers another important tool for the assessment of biological function and genetic pathways.

描述（由申请人提供）： 小鼠已成为遗传和癌症研究的中流，小鼠成像使科学家能够串行监测肿瘤的发展或缓解。 MU/PET的组合提供了功能生物学信息（例如肿瘤摄取，基因表达等）与MU/CT技术（为功能信息提供解剖环境）的MU/CT技术的组合已被证明是非常重要的工具。但是，与这些过程相关的辐射剂量可能很高，这限制了它们的频繁使用。虽然辐射不损害动物，但它可能会影响免疫反应机制并改变实验结果。我们假设在某些科学应用中，2D图像可能足以提供必要的生物学信息，以代替MU/CT和MU/PET提供的3D图像数据集。在此应用中，我们提出了针对小鼠和其他小动物的杂交二维成像系统的开发。该系统使用7射线投影成像与X射线投影射线照相相结合，我们将其称为MINX（使用核素和X射线进行鼠标成像）。 MINX系统使用单个计算的X射线照相CR检测器（BAFBR成像板）捕获7射线和X射线图像。我们假设MINX系统将为小鼠研究人员提供类似于MU/PET/MU/CT的信息，但是降低的辐射剂量和降低的成像成本将使研究人员能够对同一动物进行更多成像，从而更频繁地收集序列数据。 The six Specific Aims include (1) Monte Carlo and numerical simulation studies to define the most optimal MINX system design, (2) the manufacture of a parallel hole collimator appropriate for mouse imaging using microlithography techniques, (3) the construction of the MINX system itself, (4) the development of appropriate phantoms followed by phantom imaging, (5) initial studies on mice for further optimization, and finally (6) serial analysis using the MINX system on与MU/CT/MU/PET成像同时进行比较的小鼠。这项研究旨在证明小鼠研究中的受基金对齐的2-D核/X射线投影图像的实用性，我们希望MINX系统的发展将为小鼠研究人员提供另一个重要的工具，用于评估生物学功能和遗传途径。