Dark-Field Hard-X-ray Microscope

暗场硬 X 射线显微镜

• 批准号: 6884925
• 负责人: Melvin Arthur Piestrup
• 金额: $ 10万
• 依托单位: ADELPHI TECHNOLOGY, INC.
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-30 至 2006-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6884925
• 关键词: X ray microscopy; bioengineering /biomedical engineering; bioimaging /biomedical imaging; biomedical equipment development; contrast media; gold; image enhancement; molecular /cellular imaging; optics; phantom model; radiation dosage; tomography

DESCRIPTION (provided by applicant): This Small Business Innovation Research Phase 1 project will develop a hard-x-ray microscope capable of producing dark-field images for the detection of colloidal gold particles. Gold colloidal particles are used as labels in the study of antigens and cell and protein structure. In prior research, Adelphi Technology Inc. has developed compound refractive lenses for use in an x-ray microscope that has been used to image biological material in the bright-field mode. A prototype dark-field microscope will be developed by modifying this existing microscope. The potential for successful development of the prototype imaging system is very high because prior work by other researchers using synchrotron sources that have demonstrated the ability to detect gold particle sizes down to 20 nm in very thin specimens. The use of harder x-rays promises detection of smaller size particles inside thicker specimens and the possibility of the use of tomography for volume analysis. Replacing the synchrotron with a conventional x-ray tube as the x-ray source permits commercial and scientific use outside national laboratories. Issues to be explored in Phase I include: adequacy of x-ray source intensity, dose to specimen, expected contrast, and resolution.

描述（由申请人提供）：这个小型企业创新研究阶段1项目将开发一个硬X射线显微镜，能够生成深色图像以检测胶体金颗粒。金胶体颗粒在抗原，细胞和蛋白质结构的研究中用作标签。在先前的研究中，阿德菲技术公司（Adelphi Technology Inc.通过修改该现有显微镜，将开发一个原型的暗场显微镜。成功开发原型成像系统的潜力很高，因为其他研究人员使用同步源的工作已经证明了在非常薄的标本中检测到降低到20 nm的能力。较硬的X射线的使用有望在较厚的样品内检测较小尺寸的颗粒，并可能使用层析成像进行体积分析。 X射线源允许在国家实验室以外的商业和科学用途，用常规的X射线管代替同步器。在第一阶段探索的问题包括：X射线源强度的充分性，对标本，预期对比和分辨率的适当性。