Pattern formation during Arabidopsis embryogenesis

拟南芥胚胎发生过程中的模式形成

• 批准号: 6737995
• 负责人: NICHOLAS J KAPLINSKY
• 金额: $ 4.3万
• 依托单位: CARNEGIE INSTITUTION OF WASHINGTON, D.C.
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-01-01 至 2006-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6737995
• 关键词: Arabidopsis; cell proliferation; developmental genetics; embryogenesis; immunoprecipitation; mutant; optics; phenotype; plant genetics; plant proteins; postdoctoral investigator; protein protein interaction; protein structure function

In plants, the meristem is a group of undifferentiated stem cells that gives rise to all organs. BOBBER (BOB) is a gene responsible for the correct expression of SHOOTMERISTEMLESS (STM), a homeodomain gene required for the establishment and maintenance of Arabidopsis meristems, bob mutants show alterations in the spatial localization of STM during embryogenesis. The cloning of BOB identified a NudC domain in the BOB protein. NudC domains are evolutionarily conserved across phyla and play roles in nuclear and cell migration, secretion, and cell proliferation. Investigation of the cellular, molecular, and genetic mechanisms of BOB function will be undertaken to understand the role of this gene in developmental pattern formation. Other early patterning mutants will be identified using a novel enhancer trapping experiment. These investigations will generate useful knowledge that will contribute to an understanding of the conserved roles of NudC domains in patterning, the regulation of cell division, and differentiation. NudC domains have been implicated to play a role in cancer, and these investigations will contribute to a deeper understanding of these processes.

在植物中，分生组织是一组未分化的干细胞，产生所有器官。 BOBBER (BOB) 是负责 SHOOTMERISTEMLESS (STM) 正确表达的基因，STM 是拟南芥分生组织建立和维持所需的同源域基因，bob 突变体在胚胎发生过程中显示 STM 空间定位的改变。 BOB 的克隆鉴定出 BOB 蛋白中的 NudC 结构域。 NudC 结构域在各门中进化上是保守的，并在细胞核和细胞迁移、分泌和细胞增殖中发挥作用。将研究 BOB 功能的细胞、分子和遗传机制，以了解该基因在发育模式形成中的作用。 其他早期模式突变体将使用新型增强子捕获实验来鉴定。这些研究将产生有用的知识，有助于理解 NudC 结构域在模式形成、细胞分裂和分化调节中的保守作用。 NudC 结构域已被认为在癌症中发挥作用，这些研究将有助于更深入地了解这些过程。