Cell Fate Decision by CLV1 in Shoot Apical Meristem

CLV1 在芽顶端分生组织中决定细胞命运

• 批准号: 7109164
• 负责人: XIANG QU
• 金额: $ 4.88万
• 依托单位: CALIFORNIA INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-08-01 至 2008-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7109164
• 关键词: Arabidopsis; biological signal transduction; cell cycle; cell differentiation; cell growth regulation; cell proliferation; developmental genetics; enzyme activity; gene expression; gene mutation; plant genetics; plant growth /development; plant physiology; plant proteins; postdoctoral investigator; protein protein interaction; protein structure function; protein tyrosine kinase; tissue /cell culture

DESCRIPTION (provided by applicant): Located at the growing tip of stems, shoot apical meristems (SAMs) are actively dividing, embryonic tissues responsible for all the aerial organs in plants. It is crucial for plants to achieve a balance between cell division and cell differentiation to maintain a functional meristem. In Arabidopsis, this balance is regulated by the CLAVATA (CLV) genes. Single loss-of-function mutations of the CLVgenes result in a progressive enlargement of shoot and floral meristems. CLV1 encodes a receptor-like kinase. CLV3 encodes a small extracellular protein and appears to be a ligand of CLV1. CLV2, a receptor-like protein, may form heterodimer with CLV1. The overall project of this proposal is to examine the hypothesis that the CLV1 signaling cascade controls the proliferation and differentiation of stem cells within the SAM in Arabidopsis. To understand in molecular detail how the receptor-like kinase CLV1 functions as a key element to maintain a functional SAM, I propose to determine if CLV1 functions as a receptor at the plasma membrane; to determine the immediate and long-term effects of CLV1 on both cell division patterning and meristematic reorganization; and to identify signaling components within the CLV1 signaling pathway.

描述（由申请人提供）：茎尖分生组织（SAM）位于茎的生长尖端，是活跃分裂的胚胎组织，负责植物中的所有气生器官。对于植物来说，实现细胞分裂和细胞分化之间的平衡以维持功能性分生组织至关重要。在拟南芥中，这种平衡由 CLAVATA (CLV) 基因调节。 CLV基因的单一功能丧失突变导致芽和花分生组织逐渐增大。 CLV1 编码受体样激酶。 CLV3 编码一种小的细胞外蛋白，似乎是 CLV1 的配体。 CLV2是一种受体样蛋白，可能与CLV1形成异二聚体。该提案的总体项目是检验 CLV1 信号级联控制拟南芥 SAM 内干细胞增殖和分化的假设。为了从分子细节上了解受体样激酶 CLV1 如何作为维持 SAM 功能的关键元件发挥作用，我建议确定 CLV1 是否作为质膜上的受体发挥作用；确定 CLV1 对细胞分裂模式和分生组织重组的即时和长期影响；并鉴定 CLV1 信号通路内的信号成分。