CORE--CONFOCAL IMAGING

核心——共焦成像

• 批准号: 6441425
• 负责人: KAMIAR MOIN
• 金额: $ 8.63万
• 依托单位: WAYNE STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-04-01 至 2002-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6441425
• 关键词: biomedical facility; confocal scanning microscopy; fluorescence microscopy; immunocytochemistry; in situ hybridization; intracellular transport; light microscopy; protein transport

Description: (Applicant's Description) The purpose of the Confocal Microscopy Core is to enhance the peer-reviewed funded research activities of CCC members whose research requires confocal microscopy and related techniques. Capabilities and services include: High resolution conventional light microscopy, in situ hybridization, cytochemistry, histochemistry, immunocytochemistry, immunohistochemistry, intracellular trafficking studies, multiple labeling studies (up to 3 different parameters can be studied at the same time) and ratioing (e.g., intracellular pH and ion measurement studies), as well as three dimensional image reconstruction and quantitative measurements. The confocal imaging system consists of a Zeiss LSM 310 confocal microscope equipped with three lasers and its SiliconGraphics workstation, a Meridian ACAS 570 Analytical Cytometer equipped with one laser (tunable to three wavelengths) and a separate work station, and a Meridian InsightPlus confocal microscope also equipped with one laser. The facility also provides conventional fluorescent microscopy through a Zeiss Axiophote Dual-Camera Photomicroscope. In addition to the SiliconGraphics and the Meridian workstations, the analytical systems of the Confocal Microscopy Core include two Macintosh G3 computers with several photographic quality printers as well as various types of analytical software.

描述：（申请人的描述）共聚焦的目的 显微镜核心是为了加强同行评审资助的研究活动 研究需要共聚焦显微镜及相关技术的 CCC 成员 技术。能力和服务包括： 高分辨率传统 光学显微镜、原位杂交、细胞化学、组织化学、 免疫细胞化学、免疫组织化学、细胞内运输研究、 多重标记研究（最多可以研究 3 个不同的参数 同时）和配比（例如，细胞内 pH 和离子测量研究）， 以及三维图像重建和定量 测量。 共焦成像系统由 Zeiss LSM 310 组成 配备三个激光器的共焦显微镜及其 SiliconGraphics 工作站，配备一台激光器的 Meridian ACAS 570 分析细胞仪 （可调谐到三个波长）和一个单独的工作站，以及一个 Meridian InsightPlus 共焦显微镜还配备了一台激光器。 设施 还通过蔡司 Axiophote 提供传统荧光显微镜 双摄像头光学显微镜。 除了 SiliconGraphics 和 Meridian 工作站、共焦显微镜核心的分析系统 包括两台 Macintosh G3 计算机以及多台照相质量打印机 以及各类分析软件。